Mucoadhesive chitosan- and cellulose derivative-based nanofiber-on-foam-on-film system for non-invasive peptide delivery
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Mucoadhesive chitosan- and cellulose derivative-based nanofiber-on-foam-on-film system for non-invasive peptide delivery. / Stie, Mai Bay; Öblom, Heidi; Hansen, Anders Christian Nørgaard; Jacobsen, Jette; Chronakis, Ioannis S.; Rantanen, Jukka; Nielsen, Hanne Mørck; Genina, Natalja.
In: Carbohydrate Polymers, Vol. 303, 120429, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Mucoadhesive chitosan- and cellulose derivative-based nanofiber-on-foam-on-film system for non-invasive peptide delivery
AU - Stie, Mai Bay
AU - Öblom, Heidi
AU - Hansen, Anders Christian Nørgaard
AU - Jacobsen, Jette
AU - Chronakis, Ioannis S.
AU - Rantanen, Jukka
AU - Nielsen, Hanne Mørck
AU - Genina, Natalja
N1 - Funding Information: MBS, HMN, ISC and JJ thank The Danish Council for Independent Research; Technology and Production (DFF-6111-00333) (MBS) and University of Copenhagen for funding this project. MBS and HMN furthermore thank the Novo Nordisk Foundation (Grand Challenge Program; NNF16OC0021948). HÖ, JR and NG acknowledge Nordic POP NordForsk Program Nordic University Hub (Project #85352, Nordic POP, Patient Oriented Products). Department of Experimental Medicine at University of Copenhagen and Department of Veterinary Clinical Sciences are greatly acknowledged for providing porcine tissue for the mucoadhesion and ex vivo studies. The authors acknowledge the Core Facility for Integrated Microscopy, Faculty of Health and Medical Sciences, University of Copenhagen. Associate Professor Christian Janfelt is acknowledged for support with LC-MS analysis. Xin Zhou is acknowledged for assistance with the high-resolution SEM images of the cross-sections and the electrospun nanofibers. The authors thank LEO Pharma (Ballerup, Denmark) for availability of the dermatome equipment. Funding Information: MBS, HMN, ISC and JJ thank The Danish Council for Independent Research ; Technology and Production ( DFF-6111-00333 ) (MBS) and University of Copenhagen for funding this project. MBS and HMN furthermore thank the Novo Nordisk Foundation (Grand Challenge Program; NNF16OC0021948 ). HÖ, JR and NG acknowledge Nordic POP NordForsk Program Nordic University Hub (Project # 85352 , Nordic POP, Patient Oriented Products). Department of Experimental Medicine at University of Copenhagen and Department of Veterinary Clinical Sciences are greatly acknowledged for providing porcine tissue for the mucoadhesion and ex vivo studies. The authors acknowledge the Core Facility for Integrated Microscopy, Faculty of Health and Medical Sciences, University of Copenhagen. Associate Professor Christian Janfelt is acknowledged for support with LC-MS analysis. Xin Zhou is acknowledged for assistance with the high-resolution SEM images of the cross-sections and the electrospun nanofibers. The authors thank LEO Pharma (Ballerup, Denmark) for availability of the dermatome equipment. Publisher Copyright: © 2022 The Authors
PY - 2023
Y1 - 2023
N2 - Oromucosal administration is an attractive non-invasive route. However, drug absorption is challenged by salivary flow and the mucosa being a significant permeability barrier. The aim of this study was to design and investigate a multi-layered nanofiber-on-foam-on-film (NFF) drug delivery system with unique properties and based on polysaccharides combined as i) mucoadhesive chitosan-based nanofibers, ii) a peptide loaded hydroxypropyl methylcellulose foam, and iii) a saliva-repelling backing film based on ethylcellulose. NFF displays optimal mechanical properties shown by dynamic mechanical analysis, and biocompatibility demonstrated after exposure to a TR146 cell monolayer. Chitosan-based nanofibers provided the NFF with improved mucoadhesion compared to that of the foam alone. After 1 h, >80 % of the peptide desmopressin was released from the NFF. Ex vivo permeation studies across porcine buccal mucosa indicated that NFF improved the permeation of desmopressin compared to a commercial freeze-dried tablet. The findings demonstrate the potential of the NFF as a biocompatible drug delivery system.
AB - Oromucosal administration is an attractive non-invasive route. However, drug absorption is challenged by salivary flow and the mucosa being a significant permeability barrier. The aim of this study was to design and investigate a multi-layered nanofiber-on-foam-on-film (NFF) drug delivery system with unique properties and based on polysaccharides combined as i) mucoadhesive chitosan-based nanofibers, ii) a peptide loaded hydroxypropyl methylcellulose foam, and iii) a saliva-repelling backing film based on ethylcellulose. NFF displays optimal mechanical properties shown by dynamic mechanical analysis, and biocompatibility demonstrated after exposure to a TR146 cell monolayer. Chitosan-based nanofibers provided the NFF with improved mucoadhesion compared to that of the foam alone. After 1 h, >80 % of the peptide desmopressin was released from the NFF. Ex vivo permeation studies across porcine buccal mucosa indicated that NFF improved the permeation of desmopressin compared to a commercial freeze-dried tablet. The findings demonstrate the potential of the NFF as a biocompatible drug delivery system.
KW - Biopharmaceuticals
KW - Chitosan
KW - Hydroxypropyl methylcellulose
KW - Mucoadhesion
KW - Oromucosal drug delivery
KW - Peptide
U2 - 10.1016/j.carbpol.2022.120429
DO - 10.1016/j.carbpol.2022.120429
M3 - Journal article
C2 - 36657829
AN - SCOPUS:85144048666
VL - 303
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
SN - 0144-8617
M1 - 120429
ER -
ID: 332678252