Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery. / Davidsen, J.; Vermehren, C.; Frøkjær, S.; Mouritsen, O. G.; Jørgensen, K.

In: Advances in Colloid and Interface Science, Vol. 89-90, 29.01.2001, p. 303-311.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Davidsen, J, Vermehren, C, Frøkjær, S, Mouritsen, OG & Jørgensen, K 2001, 'Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery', Advances in Colloid and Interface Science, vol. 89-90, pp. 303-311. https://doi.org/10.1016/S0001-8686(00)00058-0

APA

Davidsen, J., Vermehren, C., Frøkjær, S., Mouritsen, O. G., & Jørgensen, K. (2001). Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery. Advances in Colloid and Interface Science, 89-90, 303-311. https://doi.org/10.1016/S0001-8686(00)00058-0

Vancouver

Davidsen J, Vermehren C, Frøkjær S, Mouritsen OG, Jørgensen K. Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery. Advances in Colloid and Interface Science. 2001 Jan 29;89-90:303-311. https://doi.org/10.1016/S0001-8686(00)00058-0

Author

Davidsen, J. ; Vermehren, C. ; Frøkjær, S. ; Mouritsen, O. G. ; Jørgensen, K. / Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery. In: Advances in Colloid and Interface Science. 2001 ; Vol. 89-90. pp. 303-311.

Bibtex

@article{92655f05bb5f4acdbda344cca28825ab,
title = "Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery",
abstract = "Polyethylenoxide (PEG) covered liposomes are used as lipid-based drug-delivery systems. In comparison to conventional liposomes the polymer-covered liposomes display a long circulation half-life in the blood stream. We investigate the influence of polyethyleneoxide-distearoylphosphatidylethanolamine (DSPE-PEG750) lipopolymer concentration on phospholipase A2 (PLA2) catalyzed hydrolysis of liposomes composed of stearoyloleoylphosphatidylcholine (SOPC). The characteristic PLA2 lag-time was determined by fluorescence and the degree of lipid hydrolysis was followed by HPLC analysis. Particle size and zeta-potential were measured as a function of DSPE-PEG750 lipopolymer concentration. A significant decrease in the lag-time, and hence an increase in enzyme activity, was observed with increasing concentrations of the anionic DSPE-PEG750 lipopolymer lipids. The observed decrease in lag-time might be related to changes in the surface potential and the PLA2 lipid membrane affinity.",
author = "J. Davidsen and C. Vermehren and S. Fr{\o}kj{\ae}r and Mouritsen, {O. G.} and K. J{\o}rgensen",
year = "2001",
month = jan,
day = "29",
doi = "10.1016/S0001-8686(00)00058-0",
language = "English",
volume = "89-90",
pages = "303--311",
journal = "Advances in Colloid and Interface Science",
issn = "0001-8686",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery

AU - Davidsen, J.

AU - Vermehren, C.

AU - Frøkjær, S.

AU - Mouritsen, O. G.

AU - Jørgensen, K.

PY - 2001/1/29

Y1 - 2001/1/29

N2 - Polyethylenoxide (PEG) covered liposomes are used as lipid-based drug-delivery systems. In comparison to conventional liposomes the polymer-covered liposomes display a long circulation half-life in the blood stream. We investigate the influence of polyethyleneoxide-distearoylphosphatidylethanolamine (DSPE-PEG750) lipopolymer concentration on phospholipase A2 (PLA2) catalyzed hydrolysis of liposomes composed of stearoyloleoylphosphatidylcholine (SOPC). The characteristic PLA2 lag-time was determined by fluorescence and the degree of lipid hydrolysis was followed by HPLC analysis. Particle size and zeta-potential were measured as a function of DSPE-PEG750 lipopolymer concentration. A significant decrease in the lag-time, and hence an increase in enzyme activity, was observed with increasing concentrations of the anionic DSPE-PEG750 lipopolymer lipids. The observed decrease in lag-time might be related to changes in the surface potential and the PLA2 lipid membrane affinity.

AB - Polyethylenoxide (PEG) covered liposomes are used as lipid-based drug-delivery systems. In comparison to conventional liposomes the polymer-covered liposomes display a long circulation half-life in the blood stream. We investigate the influence of polyethyleneoxide-distearoylphosphatidylethanolamine (DSPE-PEG750) lipopolymer concentration on phospholipase A2 (PLA2) catalyzed hydrolysis of liposomes composed of stearoyloleoylphosphatidylcholine (SOPC). The characteristic PLA2 lag-time was determined by fluorescence and the degree of lipid hydrolysis was followed by HPLC analysis. Particle size and zeta-potential were measured as a function of DSPE-PEG750 lipopolymer concentration. A significant decrease in the lag-time, and hence an increase in enzyme activity, was observed with increasing concentrations of the anionic DSPE-PEG750 lipopolymer lipids. The observed decrease in lag-time might be related to changes in the surface potential and the PLA2 lipid membrane affinity.

UR - http://www.scopus.com/inward/record.url?scp=0034746681&partnerID=8YFLogxK

U2 - 10.1016/S0001-8686(00)00058-0

DO - 10.1016/S0001-8686(00)00058-0

M3 - Journal article

C2 - 11215800

AN - SCOPUS:0034746681

VL - 89-90

SP - 303

EP - 311

JO - Advances in Colloid and Interface Science

JF - Advances in Colloid and Interface Science

SN - 0001-8686

ER -

ID: 236896632