Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids. / Skottvoll, Frøydis Sved; Aizenshtadt, Aleksandra; Hansen, Frederik André; Martinez, Mikel Amirola; Andersen, Jannike Mørch; Bogen, Inger Lise; Kutter, Jörg P.; Pedersen‐bjergaard, Stig; Lundanes, Elsa; Krauss, Stefan; Wilson, Steven Ray.

In: Analysis & Sensing, Vol. 2, No. 2, e202100051, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Skottvoll, FS, Aizenshtadt, A, Hansen, FA, Martinez, MA, Andersen, JM, Bogen, IL, Kutter, JP, Pedersen‐bjergaard, S, Lundanes, E, Krauss, S & Wilson, SR 2022, 'Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids', Analysis & Sensing, vol. 2, no. 2, e202100051. https://doi.org/10.1002/anse.202100051

APA

Skottvoll, F. S., Aizenshtadt, A., Hansen, F. A., Martinez, M. A., Andersen, J. M., Bogen, I. L., Kutter, J. P., Pedersen‐bjergaard, S., Lundanes, E., Krauss, S., & Wilson, S. R. (2022). Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids. Analysis & Sensing, 2(2), [e202100051]. https://doi.org/10.1002/anse.202100051

Vancouver

Skottvoll FS, Aizenshtadt A, Hansen FA, Martinez MA, Andersen JM, Bogen IL et al. Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids. Analysis & Sensing. 2022;2(2). e202100051. https://doi.org/10.1002/anse.202100051

Author

Skottvoll, Frøydis Sved ; Aizenshtadt, Aleksandra ; Hansen, Frederik André ; Martinez, Mikel Amirola ; Andersen, Jannike Mørch ; Bogen, Inger Lise ; Kutter, Jörg P. ; Pedersen‐bjergaard, Stig ; Lundanes, Elsa ; Krauss, Stefan ; Wilson, Steven Ray. / Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids. In: Analysis & Sensing. 2022 ; Vol. 2, No. 2.

Bibtex

@article{eb0076f472c741b08825501d4b45cbf3,
title = "Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids",
abstract = "This work introduces a strategy for organoid analysis - direct Electromembrane Extraction based Mass Spectrometry (dEME-MS) – for coupling liver organoids with mass spectrometry (MS). dEME-MS comprises electrophoresis of selected small molecules from a culture chamber across an oil membrane, and to a MS compatible solution. This enables clean micro-extraction of drugs and their metabolites as produced in the liver organoids to capillary liquid chromatography-mass spectrometry. Applying dEME-MS, proof-of-concept of directly measuring methadone metabolism is demonstrated on adult liver organoids. With 50 liver organoids and 1 μM methadone, methadone metabolism was monitored from 0 to 24 hours (11 time points). All analytes had <0.4 % variance in retention times with >100 measurements. dEME-MS is capable of automated and selective monitoring of drug metabolism in liver organoids, and could serve as a valuable tool for automated drug discovery efforts.",
author = "Skottvoll, {Fr{\o}ydis Sved} and Aleksandra Aizenshtadt and Hansen, {Frederik Andr{\'e}} and Martinez, {Mikel Amirola} and Andersen, {Jannike M{\o}rch} and Bogen, {Inger Lise} and Kutter, {J{\"o}rg P.} and Stig Pedersen‐bjergaard and Elsa Lundanes and Stefan Krauss and Wilson, {Steven Ray}",
year = "2022",
doi = "10.1002/anse.202100051",
language = "English",
volume = "2",
journal = "Analysis & Sensing",
issn = "2629-2742",
number = "2",

}

RIS

TY - JOUR

T1 - Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids

AU - Skottvoll, Frøydis Sved

AU - Aizenshtadt, Aleksandra

AU - Hansen, Frederik André

AU - Martinez, Mikel Amirola

AU - Andersen, Jannike Mørch

AU - Bogen, Inger Lise

AU - Kutter, Jörg P.

AU - Pedersen‐bjergaard, Stig

AU - Lundanes, Elsa

AU - Krauss, Stefan

AU - Wilson, Steven Ray

PY - 2022

Y1 - 2022

N2 - This work introduces a strategy for organoid analysis - direct Electromembrane Extraction based Mass Spectrometry (dEME-MS) – for coupling liver organoids with mass spectrometry (MS). dEME-MS comprises electrophoresis of selected small molecules from a culture chamber across an oil membrane, and to a MS compatible solution. This enables clean micro-extraction of drugs and their metabolites as produced in the liver organoids to capillary liquid chromatography-mass spectrometry. Applying dEME-MS, proof-of-concept of directly measuring methadone metabolism is demonstrated on adult liver organoids. With 50 liver organoids and 1 μM methadone, methadone metabolism was monitored from 0 to 24 hours (11 time points). All analytes had <0.4 % variance in retention times with >100 measurements. dEME-MS is capable of automated and selective monitoring of drug metabolism in liver organoids, and could serve as a valuable tool for automated drug discovery efforts.

AB - This work introduces a strategy for organoid analysis - direct Electromembrane Extraction based Mass Spectrometry (dEME-MS) – for coupling liver organoids with mass spectrometry (MS). dEME-MS comprises electrophoresis of selected small molecules from a culture chamber across an oil membrane, and to a MS compatible solution. This enables clean micro-extraction of drugs and their metabolites as produced in the liver organoids to capillary liquid chromatography-mass spectrometry. Applying dEME-MS, proof-of-concept of directly measuring methadone metabolism is demonstrated on adult liver organoids. With 50 liver organoids and 1 μM methadone, methadone metabolism was monitored from 0 to 24 hours (11 time points). All analytes had <0.4 % variance in retention times with >100 measurements. dEME-MS is capable of automated and selective monitoring of drug metabolism in liver organoids, and could serve as a valuable tool for automated drug discovery efforts.

U2 - 10.1002/anse.202100051

DO - 10.1002/anse.202100051

M3 - Journal article

VL - 2

JO - Analysis & Sensing

JF - Analysis & Sensing

SN - 2629-2742

IS - 2

M1 - e202100051

ER -

ID: 313016558