Generation of primary cultures of bovine brain endothelial cells and setup of cocultures with rat astrocytes
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Generation of primary cultures of bovine brain endothelial cells and setup of cocultures with rat astrocytes. / Helms, Hans C; Brodin, Birger.
In: Methods in molecular biology (Clifton, N.J.), Vol. 1135, 2014, p. 365-82.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Generation of primary cultures of bovine brain endothelial cells and setup of cocultures with rat astrocytes
AU - Helms, Hans C
AU - Brodin, Birger
PY - 2014
Y1 - 2014
N2 - In vitro models of the blood-brain barrier are useful tools to study blood-brain barrier function as well as drug permeation from the systemic circulation to the brain parenchyma. However, a large number of the available in vitro models fail to reflect the tightness of the in vivo blood-brain barrier. The present protocol describes the setup of an in vitro coculture model based on primary cultures of endothelial cells from bovine brain microvessels and primary cultures of rat astrocytes. The model displays a high electrical tightness and expresses blood-brain barrier marker proteins.
AB - In vitro models of the blood-brain barrier are useful tools to study blood-brain barrier function as well as drug permeation from the systemic circulation to the brain parenchyma. However, a large number of the available in vitro models fail to reflect the tightness of the in vivo blood-brain barrier. The present protocol describes the setup of an in vitro coculture model based on primary cultures of endothelial cells from bovine brain microvessels and primary cultures of rat astrocytes. The model displays a high electrical tightness and expresses blood-brain barrier marker proteins.
U2 - 10.1007/978-1-4939-0320-7_30
DO - 10.1007/978-1-4939-0320-7_30
M3 - Journal article
C2 - 24510879
VL - 1135
SP - 365
EP - 382
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -
ID: 102900737