Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus. / Nielsen, Amanda Kongstad Redke; Lilieholm-Røngren, Laura; Schmid, Benjamin; Holst, Bjørn; Brodin, Birger; Saaby, Lasse.

In: Stem Cell Research, Vol. 76, 103348, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nielsen, AKR, Lilieholm-Røngren, L, Schmid, B, Holst, B, Brodin, B & Saaby, L 2024, 'Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus', Stem Cell Research, vol. 76, 103348. https://doi.org/10.1016/j.scr.2024.103348

APA

Nielsen, A. K. R., Lilieholm-Røngren, L., Schmid, B., Holst, B., Brodin, B., & Saaby, L. (2024). Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus. Stem Cell Research, 76, [103348]. https://doi.org/10.1016/j.scr.2024.103348

Vancouver

Nielsen AKR, Lilieholm-Røngren L, Schmid B, Holst B, Brodin B, Saaby L. Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus. Stem Cell Research. 2024;76. 103348. https://doi.org/10.1016/j.scr.2024.103348

Author

Nielsen, Amanda Kongstad Redke ; Lilieholm-Røngren, Laura ; Schmid, Benjamin ; Holst, Bjørn ; Brodin, Birger ; Saaby, Lasse. / Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus. In: Stem Cell Research. 2024 ; Vol. 76.

Bibtex

@article{1e8fd18d9a9145189121a099253cd47a,
title = "Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus",
abstract = "The human MDR1 gene encodes the efflux transporter P-glycoprotein, which plays an important part of the blood-brain barrier function of brain microvascular endothelial cells (BMECs). Here, we report the generation of an iPSC line, where a construct of the human MDR1 gene was inserted into the safe-site locus AAVS1. This iPSC line (BIONi010-C-48) shows functional expression of P-gp and can further be differentiated and cultured into electrically tight BMEC-like monolayers exhibiting polarized expression of P-gp in the apical membrane.",
author = "Nielsen, {Amanda Kongstad Redke} and Laura Lilieholm-R{\o}ngren and Benjamin Schmid and Bj{\o}rn Holst and Birger Brodin and Lasse Saaby",
note = "Copyright {\textcopyright} 2024 The Authors. Published by Elsevier B.V. All rights reserved.",
year = "2024",
doi = "10.1016/j.scr.2024.103348",
language = "English",
volume = "76",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus

AU - Nielsen, Amanda Kongstad Redke

AU - Lilieholm-Røngren, Laura

AU - Schmid, Benjamin

AU - Holst, Bjørn

AU - Brodin, Birger

AU - Saaby, Lasse

N1 - Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.

PY - 2024

Y1 - 2024

N2 - The human MDR1 gene encodes the efflux transporter P-glycoprotein, which plays an important part of the blood-brain barrier function of brain microvascular endothelial cells (BMECs). Here, we report the generation of an iPSC line, where a construct of the human MDR1 gene was inserted into the safe-site locus AAVS1. This iPSC line (BIONi010-C-48) shows functional expression of P-gp and can further be differentiated and cultured into electrically tight BMEC-like monolayers exhibiting polarized expression of P-gp in the apical membrane.

AB - The human MDR1 gene encodes the efflux transporter P-glycoprotein, which plays an important part of the blood-brain barrier function of brain microvascular endothelial cells (BMECs). Here, we report the generation of an iPSC line, where a construct of the human MDR1 gene was inserted into the safe-site locus AAVS1. This iPSC line (BIONi010-C-48) shows functional expression of P-gp and can further be differentiated and cultured into electrically tight BMEC-like monolayers exhibiting polarized expression of P-gp in the apical membrane.

U2 - 10.1016/j.scr.2024.103348

DO - 10.1016/j.scr.2024.103348

M3 - Journal article

C2 - 38364505

VL - 76

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

M1 - 103348

ER -

ID: 384067102