TY - JOUR

T1 - Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro

AU - Rønholt, Stine

AU - Kristensen, Mie

AU - Nielsen, Hanne Mørck

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

PY - 2015

Y1 - 2015

N2 - There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.

AB - There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.

KW - Caco-2

KW - Cell viability

KW - Cell-penetrating peptides as carriers

KW - High-throughput screening

KW - In vitro Mucus

KW - Metabolic degradation

KW - Permeability

KW - Transepithelial drug delivery

UR - http://www.scopus.com/inward/record.url?scp=84938057092&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-2806-4_17

DO - 10.1007/978-1-4939-2806-4_17

M3 - Journal article

AN - SCOPUS:84938057092

VL - 1324

SP - 261

EP - 277

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -