Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery. / Panou, Danai Anastasia; Diedrichsen, Ragna Guldsmed; Kristensen, Mie; Nielsen, Hanne Mørck.

Cell Penetrating Peptides: Methods and Protocols. ed. / Ülo Langel. Springer, 2022. p. 371-384 (Methods in Molecular Biology, Vol. 2383).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Panou, DA, Diedrichsen, RG, Kristensen, M & Nielsen, HM 2022, Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery. in Ü Langel (ed.), Cell Penetrating Peptides: Methods and Protocols. Springer, Methods in Molecular Biology, vol. 2383, pp. 371-384. https://doi.org/10.1007/978-1-0716-1752-6_24

APA

Panou, D. A., Diedrichsen, R. G., Kristensen, M., & Nielsen, H. M. (2022). Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery. In Ü. Langel (Ed.), Cell Penetrating Peptides: Methods and Protocols (pp. 371-384). Springer. Methods in Molecular Biology Vol. 2383 https://doi.org/10.1007/978-1-0716-1752-6_24

Vancouver

Panou DA, Diedrichsen RG, Kristensen M, Nielsen HM. Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery. In Langel Ü, editor, Cell Penetrating Peptides: Methods and Protocols. Springer. 2022. p. 371-384. (Methods in Molecular Biology, Vol. 2383). https://doi.org/10.1007/978-1-0716-1752-6_24

Author

Panou, Danai Anastasia ; Diedrichsen, Ragna Guldsmed ; Kristensen, Mie ; Nielsen, Hanne Mørck. / Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery. Cell Penetrating Peptides: Methods and Protocols. editor / Ülo Langel. Springer, 2022. pp. 371-384 (Methods in Molecular Biology, Vol. 2383).

Bibtex

@inbook{3d9b5ce6fa0542919130754f27c6302b,
title = "Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery",
abstract = "This chapter describes the use of cell-penetrating peptides (CPPs) as carriers for transepithelial delivery of therapeutic peptides. Assessment of transepithelial peptide permeation and the mechanisms of action that permeability enhancing drug carriers exert on the epithelium requires subtle sample preparation and analysis by orthogonal methods. Here, the preparation and use of CPP-insulin physical mixture samples including the quantification of insulin by enzyme-linked immunosorbent assay (ELISA) is described. In addition, effects of CPPs on the epithelium and its barrier properties immediately upon exposure and after a recovery period are evaluated by epithelial cell viability, transepithelial electrical resistance, immunostaining of the tight junction associated zonula occludens (ZO-1) protein, and actin cytoskeleton staining.",
author = "Panou, {Danai Anastasia} and Diedrichsen, {Ragna Guldsmed} and Mie Kristensen and Nielsen, {Hanne M{\o}rck}",
note = "The Novo Nordisk Foundation is acknowledged for supporting the Center for Biopharmaceuticals and Biobarriers in Drug Delivery (BioDelivery; Grand Challenge Program, grant number NNF16OC0021948) (D.A.P., R.G.D.).",
year = "2022",
doi = "10.1007/978-1-0716-1752-6_24",
language = "English",
isbn = "978-1-0716-1751-9",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "371--384",
editor = "{\"U}lo Langel",
booktitle = "Cell Penetrating Peptides",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery

AU - Panou, Danai Anastasia

AU - Diedrichsen, Ragna Guldsmed

AU - Kristensen, Mie

AU - Nielsen, Hanne Mørck

N1 - The Novo Nordisk Foundation is acknowledged for supporting the Center for Biopharmaceuticals and Biobarriers in Drug Delivery (BioDelivery; Grand Challenge Program, grant number NNF16OC0021948) (D.A.P., R.G.D.).

PY - 2022

Y1 - 2022

N2 - This chapter describes the use of cell-penetrating peptides (CPPs) as carriers for transepithelial delivery of therapeutic peptides. Assessment of transepithelial peptide permeation and the mechanisms of action that permeability enhancing drug carriers exert on the epithelium requires subtle sample preparation and analysis by orthogonal methods. Here, the preparation and use of CPP-insulin physical mixture samples including the quantification of insulin by enzyme-linked immunosorbent assay (ELISA) is described. In addition, effects of CPPs on the epithelium and its barrier properties immediately upon exposure and after a recovery period are evaluated by epithelial cell viability, transepithelial electrical resistance, immunostaining of the tight junction associated zonula occludens (ZO-1) protein, and actin cytoskeleton staining.

AB - This chapter describes the use of cell-penetrating peptides (CPPs) as carriers for transepithelial delivery of therapeutic peptides. Assessment of transepithelial peptide permeation and the mechanisms of action that permeability enhancing drug carriers exert on the epithelium requires subtle sample preparation and analysis by orthogonal methods. Here, the preparation and use of CPP-insulin physical mixture samples including the quantification of insulin by enzyme-linked immunosorbent assay (ELISA) is described. In addition, effects of CPPs on the epithelium and its barrier properties immediately upon exposure and after a recovery period are evaluated by epithelial cell viability, transepithelial electrical resistance, immunostaining of the tight junction associated zonula occludens (ZO-1) protein, and actin cytoskeleton staining.

U2 - 10.1007/978-1-0716-1752-6_24

DO - 10.1007/978-1-0716-1752-6_24

M3 - Book chapter

C2 - 34766302

SN - 978-1-0716-1751-9

T3 - Methods in Molecular Biology

SP - 371

EP - 384

BT - Cell Penetrating Peptides

A2 - Langel, Ülo

PB - Springer

ER -

ID: 284494254