Visualisation of penetration of topical antifungal drug substances through mycosis-infected nails by matrix-assisted laser desorption ionisation mass spectrometry imaging

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Visualisation of penetration of topical antifungal drug substances through mycosis-infected nails by matrix-assisted laser desorption ionisation mass spectrometry imaging. / Endringer Pinto, Fernanda; Bagger, Charlotte; Kunze, Gernot; Joly-Tonetti, Nicolas; Thenot, Jean-Paul; Osman-Ponchet, Hanan; Janfelt, Christian.

In: Mycoses, Vol. 63, No. 8, 2020, p. 869-875.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Endringer Pinto, F, Bagger, C, Kunze, G, Joly-Tonetti, N, Thenot, J-P, Osman-Ponchet, H & Janfelt, C 2020, 'Visualisation of penetration of topical antifungal drug substances through mycosis-infected nails by matrix-assisted laser desorption ionisation mass spectrometry imaging', Mycoses, vol. 63, no. 8, pp. 869-875. https://doi.org/10.1111/myc.13103

APA

Endringer Pinto, F., Bagger, C., Kunze, G., Joly-Tonetti, N., Thenot, J-P., Osman-Ponchet, H., & Janfelt, C. (2020). Visualisation of penetration of topical antifungal drug substances through mycosis-infected nails by matrix-assisted laser desorption ionisation mass spectrometry imaging. Mycoses, 63(8), 869-875. https://doi.org/10.1111/myc.13103

Vancouver

Endringer Pinto F, Bagger C, Kunze G, Joly-Tonetti N, Thenot J-P, Osman-Ponchet H et al. Visualisation of penetration of topical antifungal drug substances through mycosis-infected nails by matrix-assisted laser desorption ionisation mass spectrometry imaging. Mycoses. 2020;63(8):869-875. https://doi.org/10.1111/myc.13103

Author

Endringer Pinto, Fernanda ; Bagger, Charlotte ; Kunze, Gernot ; Joly-Tonetti, Nicolas ; Thenot, Jean-Paul ; Osman-Ponchet, Hanan ; Janfelt, Christian. / Visualisation of penetration of topical antifungal drug substances through mycosis-infected nails by matrix-assisted laser desorption ionisation mass spectrometry imaging. In: Mycoses. 2020 ; Vol. 63, No. 8. pp. 869-875.

Bibtex

@article{6e3d8cc588dc47bd801756dc973f2a4f,
title = "Visualisation of penetration of topical antifungal drug substances through mycosis-infected nails by matrix-assisted laser desorption ionisation mass spectrometry imaging",
abstract = "Background Matrix-assisted laser desorption ionisation mass spectrometry imaging (MALDI-MSI) is a mass spectrometry-based technique, which can be applied for compound-specific imaging of pharmaceuticals in tissues samples. MALDI-MSI technology is widely used to visualise penetration and distribution profile through different tissues but has never been used with nail tissue.Objectives This study used MALDI-MSI technology to visualise distribution profile and penetration into ex vivo human mycosis-infected toenails of three antifungal active ingredients amorolfine, ciclopirox and naftifine contained in topical onychomycosis nail treatment preparations, marketed as Loceryl(R), Ciclopoli(R) and Exoderil(R).Methods Three mycosis-infected toenails were used for each treatment condition. Six and twenty-four hours after one single topical application of antifungal drugs, excess of formulation was removed, nails were cryo-sectioned at a thickness of 20 mu m, and MALDI matrix was deposited on each nail slice. Penetration and distribution profile of amorolfine, ciclopirox and naftifine in the nails were analysed by MALDI-MSI.Results All antifungal actives have been visualised in the nail by MALDI-MSI. Ciclopirox and naftifine molecules showed a highly localised distribution in the uppermost layer of the nail plate. In comparison, amorolfine diffuses through the nail plate to the deep layers already 6 hours after application and keeps diffusing towards the lowest nail layers within 24 hours.Conclusions This study shows for the first-time distribution and penetration of certain antifungal actives into human nails using MALDI-MSI analysis. The results showed a more homogeneous distribution of amorolfine to nail and a better penetration through the infected nails than ciclopirox and naftifine.",
keywords = "amorolfine 5% nail lacquer, antifungal efficacy, ex vivo, MALDI-MSI, nail penetration, topical antifungals, NAFTIFINE, PERMEATION, DELIVERY, BARRIER",
author = "{Endringer Pinto}, Fernanda and Charlotte Bagger and Gernot Kunze and Nicolas Joly-Tonetti and Jean-Paul Thenot and Hanan Osman-Ponchet and Christian Janfelt",
year = "2020",
doi = "10.1111/myc.13103",
language = "English",
volume = "63",
pages = "869--875",
journal = "Mycoses",
issn = "0933-7407",
publisher = "Wiley-Blackwell",
number = "8",

}

RIS

TY - JOUR

T1 - Visualisation of penetration of topical antifungal drug substances through mycosis-infected nails by matrix-assisted laser desorption ionisation mass spectrometry imaging

AU - Endringer Pinto, Fernanda

AU - Bagger, Charlotte

AU - Kunze, Gernot

AU - Joly-Tonetti, Nicolas

AU - Thenot, Jean-Paul

AU - Osman-Ponchet, Hanan

AU - Janfelt, Christian

PY - 2020

Y1 - 2020

N2 - Background Matrix-assisted laser desorption ionisation mass spectrometry imaging (MALDI-MSI) is a mass spectrometry-based technique, which can be applied for compound-specific imaging of pharmaceuticals in tissues samples. MALDI-MSI technology is widely used to visualise penetration and distribution profile through different tissues but has never been used with nail tissue.Objectives This study used MALDI-MSI technology to visualise distribution profile and penetration into ex vivo human mycosis-infected toenails of three antifungal active ingredients amorolfine, ciclopirox and naftifine contained in topical onychomycosis nail treatment preparations, marketed as Loceryl(R), Ciclopoli(R) and Exoderil(R).Methods Three mycosis-infected toenails were used for each treatment condition. Six and twenty-four hours after one single topical application of antifungal drugs, excess of formulation was removed, nails were cryo-sectioned at a thickness of 20 mu m, and MALDI matrix was deposited on each nail slice. Penetration and distribution profile of amorolfine, ciclopirox and naftifine in the nails were analysed by MALDI-MSI.Results All antifungal actives have been visualised in the nail by MALDI-MSI. Ciclopirox and naftifine molecules showed a highly localised distribution in the uppermost layer of the nail plate. In comparison, amorolfine diffuses through the nail plate to the deep layers already 6 hours after application and keeps diffusing towards the lowest nail layers within 24 hours.Conclusions This study shows for the first-time distribution and penetration of certain antifungal actives into human nails using MALDI-MSI analysis. The results showed a more homogeneous distribution of amorolfine to nail and a better penetration through the infected nails than ciclopirox and naftifine.

AB - Background Matrix-assisted laser desorption ionisation mass spectrometry imaging (MALDI-MSI) is a mass spectrometry-based technique, which can be applied for compound-specific imaging of pharmaceuticals in tissues samples. MALDI-MSI technology is widely used to visualise penetration and distribution profile through different tissues but has never been used with nail tissue.Objectives This study used MALDI-MSI technology to visualise distribution profile and penetration into ex vivo human mycosis-infected toenails of three antifungal active ingredients amorolfine, ciclopirox and naftifine contained in topical onychomycosis nail treatment preparations, marketed as Loceryl(R), Ciclopoli(R) and Exoderil(R).Methods Three mycosis-infected toenails were used for each treatment condition. Six and twenty-four hours after one single topical application of antifungal drugs, excess of formulation was removed, nails were cryo-sectioned at a thickness of 20 mu m, and MALDI matrix was deposited on each nail slice. Penetration and distribution profile of amorolfine, ciclopirox and naftifine in the nails were analysed by MALDI-MSI.Results All antifungal actives have been visualised in the nail by MALDI-MSI. Ciclopirox and naftifine molecules showed a highly localised distribution in the uppermost layer of the nail plate. In comparison, amorolfine diffuses through the nail plate to the deep layers already 6 hours after application and keeps diffusing towards the lowest nail layers within 24 hours.Conclusions This study shows for the first-time distribution and penetration of certain antifungal actives into human nails using MALDI-MSI analysis. The results showed a more homogeneous distribution of amorolfine to nail and a better penetration through the infected nails than ciclopirox and naftifine.

KW - amorolfine 5% nail lacquer

KW - antifungal efficacy

KW - ex vivo

KW - MALDI-MSI

KW - nail penetration

KW - topical antifungals

KW - NAFTIFINE

KW - PERMEATION

KW - DELIVERY

KW - BARRIER

U2 - 10.1111/myc.13103

DO - 10.1111/myc.13103

M3 - Journal article

C2 - 32406142

VL - 63

SP - 869

EP - 875

JO - Mycoses

JF - Mycoses

SN - 0933-7407

IS - 8

ER -

ID: 247075479