Towards analyzing the potential of exosomes to deliver microRNA therapeutics

Research output: Contribution to journalJournal articleResearchpeer-review

  • Lefki-Pavlina N. Giassafaki
  • Scheyla Siqueira
  • Emmanuel Panteris
  • Konstantina Psatha
  • Fani Chatzopoulou
  • Michalis Aivaliotis
  • Georgios Tzimagiorgis
  • Müllertz, Anette
  • Dimitrios G. Fatouros
  • Ioannis S. Vizirianakis

Exosome selectivity mechanisms underlying exosome-target cell interactions and the specific traits affecting their capability to communicate still remain unclear. Moreover, the capacity of exosomes to efficiently deliver their molecular cargos intracellularly needs precise investigation towards establishing functional exosome-based delivery platforms exploitable in the clinical practice. The current study focuses on: (a) exosome production from normal MRC-5 and Vero cells growing in culture, (b) physicochemical characterization by dynamic light scattering (DLS) and cryo-transmission electron microscopy; (c) cellular uptake studies of rhodamine-labeled exosomes in normal and cancer cells, providing to exosomes either "autologous" or "heterologous" cellular delivery environments; and (d) loading exogenous Alexa Fluor 488-labeled siRNA into exosomes for the assessment of their delivering capacity by immunofluorescence in a panel of recipient cells. The data obtained thus far indicate that MRC-5 and Vero exosomes, indeed exhibit an interesting delivering profile, as promising "bio-shuttles," being pharmacologically exploitable in the context of theranostic applications.

Original languageEnglish
JournalJournal of Cellular Physiology
Number of pages16
ISSN0021-9541
DOIs
Publication statusE-pub ahead of print - 2020

    Research areas

  • cellular uptake selectivity, cryo-transmission electron microscopy, delivery, exosomes, siRNAs, microRNAs, EXTRACELLULAR VESICLES, MEDIATED DELIVERY, VERO CELLS, IN-VITRO, DRUG, EXTERNALIZATION, NANOTECHNOLOGY, MATURATION, RETICULOCYTES, GENOMICS

ID: 248767110