Towards analyzing the potential of exosomes to deliver microRNA therapeutics
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Towards analyzing the potential of exosomes to deliver microRNA therapeutics. / Giassafaki, Lefki-Pavlina N.; Siqueira, Scheyla; Panteris, Emmanuel; Psatha, Konstantina; Chatzopoulou, Fani; Aivaliotis, Michalis; Tzimagiorgis, Georgios; Mullertz, Anette; Fatouros, Dimitrios G.; Vizirianakis, Ioannis S.
In: Journal of Cellular Physiology, Vol. 236, No. 2, 2021, p. 1529-1544.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Towards analyzing the potential of exosomes to deliver microRNA therapeutics
AU - Giassafaki, Lefki-Pavlina N.
AU - Siqueira, Scheyla
AU - Panteris, Emmanuel
AU - Psatha, Konstantina
AU - Chatzopoulou, Fani
AU - Aivaliotis, Michalis
AU - Tzimagiorgis, Georgios
AU - Mullertz, Anette
AU - Fatouros, Dimitrios G.
AU - Vizirianakis, Ioannis S.
PY - 2021
Y1 - 2021
N2 - Exosome selectivity mechanisms underlying exosome-target cell interactions and the specific traits affecting their capability to communicate still remain unclear. Moreover, the capacity of exosomes to efficiently deliver their molecular cargos intracellularly needs precise investigation towards establishing functional exosome-based delivery platforms exploitable in the clinical practice. The current study focuses on: (a) exosome production from normal MRC-5 and Vero cells growing in culture, (b) physicochemical characterization by dynamic light scattering (DLS) and cryo-transmission electron microscopy; (c) cellular uptake studies of rhodamine-labeled exosomes in normal and cancer cells, providing to exosomes either "autologous" or "heterologous" cellular delivery environments; and (d) loading exogenous Alexa Fluor 488-labeled siRNA into exosomes for the assessment of their delivering capacity by immunofluorescence in a panel of recipient cells. The data obtained thus far indicate that MRC-5 and Vero exosomes, indeed exhibit an interesting delivering profile, as promising "bio-shuttles," being pharmacologically exploitable in the context of theranostic applications.
AB - Exosome selectivity mechanisms underlying exosome-target cell interactions and the specific traits affecting their capability to communicate still remain unclear. Moreover, the capacity of exosomes to efficiently deliver their molecular cargos intracellularly needs precise investigation towards establishing functional exosome-based delivery platforms exploitable in the clinical practice. The current study focuses on: (a) exosome production from normal MRC-5 and Vero cells growing in culture, (b) physicochemical characterization by dynamic light scattering (DLS) and cryo-transmission electron microscopy; (c) cellular uptake studies of rhodamine-labeled exosomes in normal and cancer cells, providing to exosomes either "autologous" or "heterologous" cellular delivery environments; and (d) loading exogenous Alexa Fluor 488-labeled siRNA into exosomes for the assessment of their delivering capacity by immunofluorescence in a panel of recipient cells. The data obtained thus far indicate that MRC-5 and Vero exosomes, indeed exhibit an interesting delivering profile, as promising "bio-shuttles," being pharmacologically exploitable in the context of theranostic applications.
KW - cellular uptake selectivity
KW - cryo-transmission electron microscopy
KW - delivery
KW - exosomes
KW - siRNAs
KW - microRNAs
KW - EXTRACELLULAR VESICLES
KW - MEDIATED DELIVERY
KW - VERO CELLS
KW - IN-VITRO
KW - DRUG
KW - EXTERNALIZATION
KW - NANOTECHNOLOGY
KW - MATURATION
KW - RETICULOCYTES
KW - GENOMICS
U2 - 10.1002/jcp.29991
DO - 10.1002/jcp.29991
M3 - Journal article
C2 - 32749687
VL - 236
SP - 1529
EP - 1544
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
IS - 2
ER -
ID: 248767110