Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer. / van de Weert, Marco; van Dijkhuizen-Radersma, Riemke; Bezemer, Jeroen M; Hennink, Wim E; Crommelin, Daan J A.

In: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, Vol. 54, No. 1, 07.2002, p. 89-93.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

van de Weert, M, van Dijkhuizen-Radersma, R, Bezemer, JM, Hennink, WE & Crommelin, DJA 2002, 'Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer', European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, vol. 54, no. 1, pp. 89-93.

APA

van de Weert, M., van Dijkhuizen-Radersma, R., Bezemer, J. M., Hennink, W. E., & Crommelin, D. J. A. (2002). Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 54(1), 89-93.

Vancouver

van de Weert M, van Dijkhuizen-Radersma R, Bezemer JM, Hennink WE, Crommelin DJA. Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V. 2002 Jul;54(1):89-93.

Author

van de Weert, Marco ; van Dijkhuizen-Radersma, Riemke ; Bezemer, Jeroen M ; Hennink, Wim E ; Crommelin, Daan J A. / Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer. In: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V. 2002 ; Vol. 54, No. 1. pp. 89-93.

Bibtex

@article{585ae35142e348ea8c85178a158a42b0,
title = "Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer",
abstract = "Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.",
author = "{van de Weert}, Marco and {van Dijkhuizen-Radersma}, Riemke and Bezemer, {Jeroen M} and Hennink, {Wim E} and Crommelin, {Daan J A}",
year = "2002",
month = "7",
language = "English",
volume = "54",
pages = "89--93",
journal = "European Journal of Pharmaceutics and Biopharmaceutics",
issn = "0939-6411",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer

AU - van de Weert, Marco

AU - van Dijkhuizen-Radersma, Riemke

AU - Bezemer, Jeroen M

AU - Hennink, Wim E

AU - Crommelin, Daan J A

PY - 2002/7

Y1 - 2002/7

N2 - Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.

AB - Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.

M3 - Journal article

C2 - 12084507

VL - 54

SP - 89

EP - 93

JO - European Journal of Pharmaceutics and Biopharmaceutics

JF - European Journal of Pharmaceutics and Biopharmaceutics

SN - 0939-6411

IS - 1

ER -

ID: 45184763