Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer
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Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer. / van de Weert, Marco; van Dijkhuizen-Radersma, Riemke; Bezemer, Jeroen M; Hennink, Wim E; Crommelin, Daan J A.
In: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, Vol. 54, No. 1, 07.2002, p. 89-93.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer
AU - van de Weert, Marco
AU - van Dijkhuizen-Radersma, Riemke
AU - Bezemer, Jeroen M
AU - Hennink, Wim E
AU - Crommelin, Daan J A
PY - 2002/7
Y1 - 2002/7
N2 - Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.
AB - Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.
M3 - Journal article
C2 - 12084507
VL - 54
SP - 89
EP - 93
JO - European Journal of Pharmaceutics and Biopharmaceutics
JF - European Journal of Pharmaceutics and Biopharmaceutics
SN - 0939-6411
IS - 1
ER -
ID: 45184763