Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer

Research output: Contribution to journalJournal articleResearchpeer-review

  • van de Weert, Marco
  • Riemke van Dijkhuizen-Radersma
  • Jeroen M Bezemer
  • Wim E Hennink
  • Daan J A Crommelin
Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.
Original languageEnglish
JournalEuropean journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
Issue number1
Pages (from-to)89-93
Number of pages5
Publication statusPublished - Jul 2002
Externally publishedYes

ID: 45184763