Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

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The ability to immobilize biomolecules at specific locations on the surface of solid supports is central to many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in thiol-ene microfluidic chips. Adjusting the stoichiometric ratio of "thiol" and "ene" monomers present in the microfluidic chip bulk material provides a simple and efficient way of tuning the chip's surface chemistry. Here, thiol-ene chips displaying an excess of functional thiol groups at their surfaces are functionalized with biotin and streptavidin in a controlled fashion using photolithography. We also present quantitative data on the number of functional groups available for surface modification on thiol-ene substrates and their stability.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Number of pages3
PublisherChemical and Biological Microsystems Society
Publication date1 Jan 2012
ISBN (Print)9780979806452
Publication statusPublished - 1 Jan 2012

    Research areas

  • Biochips, Biomolecule immobilization, Surface functionalization, Thiol-ene polymers

ID: 117304652