Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Standard

Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. / Lafleur, Josiane P.; Kwapiszewski, Radoslaw; Jensen, Thomas G.; Kutter, Jörg P.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1258-1260.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Harvard

Lafleur, JP, Kwapiszewski, R, Jensen, TG & Kutter, JP 2012, Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 1258-1260. <http://www.scopus.com/inward/record.url?scp=84901814641&partnerID=8YFLogxK>

APA

Lafleur, J. P., Kwapiszewski, R., Jensen, T. G., & Kutter, J. P. (2012). Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1258-1260). Chemical and Biological Microsystems Society. http://www.scopus.com/inward/record.url?scp=84901814641&partnerID=8YFLogxK

Vancouver

Lafleur JP, Kwapiszewski R, Jensen TG, Kutter JP. Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1258-1260

Author

Lafleur, Josiane P. ; Kwapiszewski, Radoslaw ; Jensen, Thomas G. ; Kutter, Jörg P. / Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 1258-1260

Bibtex

@inproceedings{b6f3483413ea496ea060442de5101df9,
title = "Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices",
abstract = "The ability to immobilize biomolecules at specific locations on the surface of solid supports is central to many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in thiol-ene microfluidic chips. Adjusting the stoichiometric ratio of {"}thiol{"} and {"}ene{"} monomers present in the microfluidic chip bulk material provides a simple and efficient way of tuning the chip's surface chemistry. Here, thiol-ene chips displaying an excess of functional thiol groups at their surfaces are functionalized with biotin and streptavidin in a controlled fashion using photolithography. We also present quantitative data on the number of functional groups available for surface modification on thiol-ene substrates and their stability.",
keywords = "Biochips, Biomolecule immobilization, Surface functionalization, Thiol-ene polymers",
author = "Lafleur, {Josiane P.} and Radoslaw Kwapiszewski and Jensen, {Thomas G.} and Kutter, {J{\"o}rg P.}",
year = "2012",
month = jan,
day = "1",
language = "English",
isbn = "9780979806452",
pages = "1258--1260",
booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
publisher = "Chemical and Biological Microsystems Society",

}

RIS

TY - GEN

T1 - Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

AU - Lafleur, Josiane P.

AU - Kwapiszewski, Radoslaw

AU - Jensen, Thomas G.

AU - Kutter, Jörg P.

PY - 2012/1/1

Y1 - 2012/1/1

N2 - The ability to immobilize biomolecules at specific locations on the surface of solid supports is central to many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in thiol-ene microfluidic chips. Adjusting the stoichiometric ratio of "thiol" and "ene" monomers present in the microfluidic chip bulk material provides a simple and efficient way of tuning the chip's surface chemistry. Here, thiol-ene chips displaying an excess of functional thiol groups at their surfaces are functionalized with biotin and streptavidin in a controlled fashion using photolithography. We also present quantitative data on the number of functional groups available for surface modification on thiol-ene substrates and their stability.

AB - The ability to immobilize biomolecules at specific locations on the surface of solid supports is central to many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in thiol-ene microfluidic chips. Adjusting the stoichiometric ratio of "thiol" and "ene" monomers present in the microfluidic chip bulk material provides a simple and efficient way of tuning the chip's surface chemistry. Here, thiol-ene chips displaying an excess of functional thiol groups at their surfaces are functionalized with biotin and streptavidin in a controlled fashion using photolithography. We also present quantitative data on the number of functional groups available for surface modification on thiol-ene substrates and their stability.

KW - Biochips

KW - Biomolecule immobilization

KW - Surface functionalization

KW - Thiol-ene polymers

M3 - Article in proceedings

AN - SCOPUS:84901814641

SN - 9780979806452

SP - 1258

EP - 1260

BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

PB - Chemical and Biological Microsystems Society

ER -

ID: 117304652