Raman mapping of mannitol/lysozyme particles produced via spray drying and single droplet drying

Research output: Contribution to journalJournal articleResearchpeer-review

Jari Pekka Pajander, Sanni Elina Matero, Jakob Sloth, Feng Wan, Jukka Rantanen, Mingshi Yang

PURPOSE: This study aimed to investigate the effect of a model protein on the solid state of a commonly used bulk agent in spray-dried formulations.

METHODS: A series of lysozyme/mannitol formulations were spray-dried using a lab-scale spray dryer. Further, the surface temperature of drying droplet/particles was monitored using the DRYING KINETICS ANALYZER™ (DKA) with controllable drying conditions mimicking the spray-drying process to estimate the drying kinetics of the lysozyme/mannitol formulations. The mannitol polymorphism and the spatial distribution of lysozyme in the particles were examined using X-ray powder diffractometry (XRPD) and Raman microscopy. Partial Least Squares Discriminant Analysis was used for analyzing the Raman microscopy data.

RESULTS: XRPD results indicated that a mixture of β-mannitol and α-mannitol was produced in the spray-drying process which was supported by the Raman analysis, whereas Raman analysis indicated that a mixture of α-mannitol and δ-mannitol was detected in the single particles from DKA. In addition Raman mapping indicated that the presence of lysozyme seemed to favor the appearance of α-mannitol in the particles from DKA evidenced by close proximity of lysozyme and mannitol in the particles.

CONCLUSIONS: It suggested that the presence of lysozyme tend to induce metastable solid state forms upon the drying process.

Original languageEnglish
JournalPharmaceutical Research
Issue number6
Pages (from-to)1993-2002
Number of pages10
Publication statusPublished - 2015

ID: 162897994