Raman mapping of mannitol/lysozyme particles produced via spray drying and single droplet drying

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Raman mapping of mannitol/lysozyme particles produced via spray drying and single droplet drying. / Pekka Pajander, Jari; Matero, Sanni Elina; Sloth, Jakob; Wan, Feng; Rantanen, Jukka; Yang, Mingshi.

In: Pharmaceutical Research, Vol. 32, No. 6, 2015, p. 1993-2002.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Pekka Pajander, J, Matero, SE, Sloth, J, Wan, F, Rantanen, J & Yang, M 2015, 'Raman mapping of mannitol/lysozyme particles produced via spray drying and single droplet drying', Pharmaceutical Research, vol. 32, no. 6, pp. 1993-2002. https://doi.org/10.1007/s11095-014-1592-z

APA

Pekka Pajander, J., Matero, S. E., Sloth, J., Wan, F., Rantanen, J., & Yang, M. (2015). Raman mapping of mannitol/lysozyme particles produced via spray drying and single droplet drying. Pharmaceutical Research, 32(6), 1993-2002. https://doi.org/10.1007/s11095-014-1592-z

Vancouver

Pekka Pajander J, Matero SE, Sloth J, Wan F, Rantanen J, Yang M. Raman mapping of mannitol/lysozyme particles produced via spray drying and single droplet drying. Pharmaceutical Research. 2015;32(6):1993-2002. https://doi.org/10.1007/s11095-014-1592-z

Author

Pekka Pajander, Jari ; Matero, Sanni Elina ; Sloth, Jakob ; Wan, Feng ; Rantanen, Jukka ; Yang, Mingshi. / Raman mapping of mannitol/lysozyme particles produced via spray drying and single droplet drying. In: Pharmaceutical Research. 2015 ; Vol. 32, No. 6. pp. 1993-2002.

Bibtex

@article{d518096e45ef44f582869ec13ec0fc11,
title = "Raman mapping of mannitol/lysozyme particles produced via spray drying and single droplet drying",
abstract = "PURPOSE: This study aimed to investigate the effect of a model protein on the solid state of a commonly used bulk agent in spray-dried formulations.METHODS: A series of lysozyme/mannitol formulations were spray-dried using a lab-scale spray dryer. Further, the surface temperature of drying droplet/particles was monitored using the DRYING KINETICS ANALYZER™ (DKA) with controllable drying conditions mimicking the spray-drying process to estimate the drying kinetics of the lysozyme/mannitol formulations. The mannitol polymorphism and the spatial distribution of lysozyme in the particles were examined using X-ray powder diffractometry (XRPD) and Raman microscopy. Partial Least Squares Discriminant Analysis was used for analyzing the Raman microscopy data.RESULTS: XRPD results indicated that a mixture of β-mannitol and α-mannitol was produced in the spray-drying process which was supported by the Raman analysis, whereas Raman analysis indicated that a mixture of α-mannitol and δ-mannitol was detected in the single particles from DKA. In addition Raman mapping indicated that the presence of lysozyme seemed to favor the appearance of α-mannitol in the particles from DKA evidenced by close proximity of lysozyme and mannitol in the particles.CONCLUSIONS: It suggested that the presence of lysozyme tend to induce metastable solid state forms upon the drying process.",
author = "{Pekka Pajander}, Jari and Matero, {Sanni Elina} and Jakob Sloth and Feng Wan and Jukka Rantanen and Mingshi Yang",
year = "2015",
doi = "10.1007/s11095-014-1592-z",
language = "English",
volume = "32",
pages = "1993--2002",
journal = "Pharmaceutical Research",
issn = "0724-8741",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - Raman mapping of mannitol/lysozyme particles produced via spray drying and single droplet drying

AU - Pekka Pajander, Jari

AU - Matero, Sanni Elina

AU - Sloth, Jakob

AU - Wan, Feng

AU - Rantanen, Jukka

AU - Yang, Mingshi

PY - 2015

Y1 - 2015

N2 - PURPOSE: This study aimed to investigate the effect of a model protein on the solid state of a commonly used bulk agent in spray-dried formulations.METHODS: A series of lysozyme/mannitol formulations were spray-dried using a lab-scale spray dryer. Further, the surface temperature of drying droplet/particles was monitored using the DRYING KINETICS ANALYZER™ (DKA) with controllable drying conditions mimicking the spray-drying process to estimate the drying kinetics of the lysozyme/mannitol formulations. The mannitol polymorphism and the spatial distribution of lysozyme in the particles were examined using X-ray powder diffractometry (XRPD) and Raman microscopy. Partial Least Squares Discriminant Analysis was used for analyzing the Raman microscopy data.RESULTS: XRPD results indicated that a mixture of β-mannitol and α-mannitol was produced in the spray-drying process which was supported by the Raman analysis, whereas Raman analysis indicated that a mixture of α-mannitol and δ-mannitol was detected in the single particles from DKA. In addition Raman mapping indicated that the presence of lysozyme seemed to favor the appearance of α-mannitol in the particles from DKA evidenced by close proximity of lysozyme and mannitol in the particles.CONCLUSIONS: It suggested that the presence of lysozyme tend to induce metastable solid state forms upon the drying process.

AB - PURPOSE: This study aimed to investigate the effect of a model protein on the solid state of a commonly used bulk agent in spray-dried formulations.METHODS: A series of lysozyme/mannitol formulations were spray-dried using a lab-scale spray dryer. Further, the surface temperature of drying droplet/particles was monitored using the DRYING KINETICS ANALYZER™ (DKA) with controllable drying conditions mimicking the spray-drying process to estimate the drying kinetics of the lysozyme/mannitol formulations. The mannitol polymorphism and the spatial distribution of lysozyme in the particles were examined using X-ray powder diffractometry (XRPD) and Raman microscopy. Partial Least Squares Discriminant Analysis was used for analyzing the Raman microscopy data.RESULTS: XRPD results indicated that a mixture of β-mannitol and α-mannitol was produced in the spray-drying process which was supported by the Raman analysis, whereas Raman analysis indicated that a mixture of α-mannitol and δ-mannitol was detected in the single particles from DKA. In addition Raman mapping indicated that the presence of lysozyme seemed to favor the appearance of α-mannitol in the particles from DKA evidenced by close proximity of lysozyme and mannitol in the particles.CONCLUSIONS: It suggested that the presence of lysozyme tend to induce metastable solid state forms upon the drying process.

U2 - 10.1007/s11095-014-1592-z

DO - 10.1007/s11095-014-1592-z

M3 - Journal article

VL - 32

SP - 1993

EP - 2002

JO - Pharmaceutical Research

JF - Pharmaceutical Research

SN - 0724-8741

IS - 6

ER -

ID: 162897994