Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine
Research output: Contribution to journal › Journal article › Research › peer-review
A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesisof the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-l-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1±25.2% of the dose.
|Journal||Journal of Chromatography - B|
|Number of pages||6|
|Publication status||Published - 2007|
- Former LIFE faculty - Isothiocyantes, Biomarker, Synthesis, 1,3-Benzodithiol e-2-thione, N-acetyl-S(N4-methylsulfinylbutythihocarbam oyl)4-cystione