Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine

Research output: Contribution to journal › Journal article › peer-review

Standard

Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine. / Kristensen, Mette; Krogholm, Kirstine S.; Frederiksen, Hanne; Duus, Fritz; Cornett, Claus; Bügel, Susanne Gjedsted; Rasmussen, Salka E.

In: Journal of Chromatography - B, Vol. 852, No. 1-2, 2007, p. 229-234.

Research output: Contribution to journal › Journal article › peer-review

Harvard

Kristensen, M, Krogholm, KS, Frederiksen, H, Duus, F, Cornett, C, Bügel, SG & Rasmussen, SE 2007, 'Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine', Journal of Chromatography - B, vol. 852, no. 1-2, pp. 229-234. https://doi.org/10.1016/j.jchromb.2007.01.022

APA

Kristensen, M., Krogholm, K. S., Frederiksen, H., Duus, F., Cornett, C., Bügel, S. G., & Rasmussen, S. E. (2007). Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine. Journal of Chromatography - B, 852(1-2), 229-234. https://doi.org/10.1016/j.jchromb.2007.01.022

Vancouver

Kristensen M, Krogholm KS, Frederiksen H, Duus F, Cornett C, Bügel SG et al. Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine. Journal of Chromatography - B. 2007;852(1-2):229-234. https://doi.org/10.1016/j.jchromb.2007.01.022

Author

Kristensen, Mette ; Krogholm, Kirstine S. ; Frederiksen, Hanne ; Duus, Fritz ; Cornett, Claus ; Bügel, Susanne Gjedsted ; Rasmussen, Salka E. / Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine. In: Journal of Chromatography - B. 2007 ; Vol. 852, No. 1-2. pp. 229-234.

Bibtex

@article{6aeeb8a0a1c211ddb6ae000ea68e967b,
title = "Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine",
abstract = "A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesisof the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-l-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1±25.2% of the dose.",
keywords = "Former LIFE faculty, Isothiocyantes, Biomarker, Synthesis, 1,3-Benzodithiol e-2-thione, N-acetyl-S(N4-methylsulfinylbutythihocarbam oyl)4-cystione",
author = "Mette Kristensen and Krogholm, {Kirstine S.} and Hanne Frederiksen and Fritz Duus and Claus Cornett and B{\"u}gel, {Susanne Gjedsted} and Rasmussen, {Salka E.}",
year = "2007",
doi = "10.1016/j.jchromb.2007.01.022",
language = "English",
volume = "852",
pages = "229--234",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine

AU - Kristensen, Mette

AU - Krogholm, Kirstine S.

AU - Frederiksen, Hanne

AU - Duus, Fritz

AU - Cornett, Claus

AU - Bügel, Susanne Gjedsted

AU - Rasmussen, Salka E.

PY - 2007

Y1 - 2007

N2 - A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesisof the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-l-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1±25.2% of the dose.

AB - A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesisof the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-l-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1±25.2% of the dose.

KW - Former LIFE faculty

KW - Isothiocyantes

KW - Biomarker

KW - Synthesis

KW - 1,3-Benzodithiol e-2-thione

KW - N-acetyl-S(N4-methylsulfinylbutythihocarbam oyl)4-cystione

U2 - 10.1016/j.jchromb.2007.01.022

DO - 10.1016/j.jchromb.2007.01.022

M3 - Journal article

C2 - 17307405

VL - 852

SP - 229

EP - 234

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 1-2

ER -

ID: 8070819