An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium

Research output: Contribution to journalJournal articleResearchpeer-review

The aim of this study was to investigate the mechanisms by which increases in free cytosolic calcium ([Ca2+]i) cause a decrease in macroscopic sodium absorption across principal cells of the frog skin epithelium. [Ca2+]i was measured with fura-2 in an epifluorescence microscope set-up, sodium absorption was measured by the voltage-clamp technique and cellular potential was measured using microelectrodes. The endoplasmic reticulum calcium-ATPase inhibitor thapsigargin (0.4 microM) increased [Ca2+]i from 66 +/- 9 to 137 +/- 19 nM (n = 13, P = 0.002). Thapsigargin caused the amiloride-sensitive short circuit current (Isc) to drop from 26.4 to 10.6 microA cm-2 (n = 19, P
Original languageEnglish
JournalPflügers Archiv - European Journal of Physiology
Issue number1-2
Pages (from-to)16-25
Number of pages10
Publication statusPublished - 1996

    Research areas

  • Animals, Biological Transport, Active, Calcium, Cell Membrane, Chloride Channels, Chlorides, Cytosol, Electrophysiology, Epithelium, Female, Intracellular Membranes, Male, Osmolar Concentration, Permeability, Potassium, Rana temporaria, Skin, Sodium, Sodium Channel Blockers, Thapsigargin

ID: 37899870