An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium. / Brodin, Birger; Rytved, K A; Nielsen, R.

In: Pflügers Archiv - European Journal of Physiology, Vol. 433, No. 1-2, 1996, p. 16-25.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Brodin, B, Rytved, KA & Nielsen, R 1996, 'An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium', Pflügers Archiv - European Journal of Physiology, vol. 433, no. 1-2, pp. 16-25.

APA

Brodin, B., Rytved, K. A., & Nielsen, R. (1996). An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium. Pflügers Archiv - European Journal of Physiology, 433(1-2), 16-25.

Vancouver

Brodin B, Rytved KA, Nielsen R. An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium. Pflügers Archiv - European Journal of Physiology. 1996;433(1-2):16-25.

Author

Brodin, Birger ; Rytved, K A ; Nielsen, R. / An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium. In: Pflügers Archiv - European Journal of Physiology. 1996 ; Vol. 433, No. 1-2. pp. 16-25.

Bibtex

@article{30ffe05899fd444383c242065e786975,
title = "An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium",
abstract = "The aim of this study was to investigate the mechanisms by which increases in free cytosolic calcium ([Ca2+]i) cause a decrease in macroscopic sodium absorption across principal cells of the frog skin epithelium. [Ca2+]i was measured with fura-2 in an epifluorescence microscope set-up, sodium absorption was measured by the voltage-clamp technique and cellular potential was measured using microelectrodes. The endoplasmic reticulum calcium-ATPase inhibitor thapsigargin (0.4 microM) increased [Ca2+]i from 66 +/- 9 to 137 +/- 19 nM (n = 13, P = 0.002). Thapsigargin caused the amiloride-sensitive short circuit current (Isc) to drop from 26.4 to 10.6 microA cm-2 (n = 19, P",
keywords = "Animals, Biological Transport, Active, Calcium, Cell Membrane, Chloride Channels, Chlorides, Cytosol, Electrophysiology, Epithelium, Female, Intracellular Membranes, Male, Osmolar Concentration, Permeability, Potassium, Rana temporaria, Skin, Sodium, Sodium Channel Blockers, Thapsigargin",
author = "Birger Brodin and Rytved, {K A} and R Nielsen",
year = "1996",
language = "English",
volume = "433",
pages = "16--25",
journal = "Pfl{\"u}gers Archiv - European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer",
number = "1-2",

}

RIS

TY - JOUR

T1 - An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium

AU - Brodin, Birger

AU - Rytved, K A

AU - Nielsen, R

PY - 1996

Y1 - 1996

N2 - The aim of this study was to investigate the mechanisms by which increases in free cytosolic calcium ([Ca2+]i) cause a decrease in macroscopic sodium absorption across principal cells of the frog skin epithelium. [Ca2+]i was measured with fura-2 in an epifluorescence microscope set-up, sodium absorption was measured by the voltage-clamp technique and cellular potential was measured using microelectrodes. The endoplasmic reticulum calcium-ATPase inhibitor thapsigargin (0.4 microM) increased [Ca2+]i from 66 +/- 9 to 137 +/- 19 nM (n = 13, P = 0.002). Thapsigargin caused the amiloride-sensitive short circuit current (Isc) to drop from 26.4 to 10.6 microA cm-2 (n = 19, P

AB - The aim of this study was to investigate the mechanisms by which increases in free cytosolic calcium ([Ca2+]i) cause a decrease in macroscopic sodium absorption across principal cells of the frog skin epithelium. [Ca2+]i was measured with fura-2 in an epifluorescence microscope set-up, sodium absorption was measured by the voltage-clamp technique and cellular potential was measured using microelectrodes. The endoplasmic reticulum calcium-ATPase inhibitor thapsigargin (0.4 microM) increased [Ca2+]i from 66 +/- 9 to 137 +/- 19 nM (n = 13, P = 0.002). Thapsigargin caused the amiloride-sensitive short circuit current (Isc) to drop from 26.4 to 10.6 microA cm-2 (n = 19, P

KW - Animals

KW - Biological Transport, Active

KW - Calcium

KW - Cell Membrane

KW - Chloride Channels

KW - Chlorides

KW - Cytosol

KW - Electrophysiology

KW - Epithelium

KW - Female

KW - Intracellular Membranes

KW - Male

KW - Osmolar Concentration

KW - Permeability

KW - Potassium

KW - Rana temporaria

KW - Skin

KW - Sodium

KW - Sodium Channel Blockers

KW - Thapsigargin

M3 - Journal article

C2 - 9019717

VL - 433

SP - 16

EP - 25

JO - Pflügers Archiv - European Journal of Physiology

JF - Pflügers Archiv - European Journal of Physiology

SN - 0031-6768

IS - 1-2

ER -

ID: 37899870