Analysis of vitamin formulations by electrokinetic chromatography utilizing tetradecylammonium ions as the pseudostationary phase
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Analysis of vitamin formulations by electrokinetic chromatography utilizing tetradecylammonium ions as the pseudostationary phase. / Næss, Øystein; Tilander, Tuija; Pedersen-Bjergaard, Stig; Rasmussen, Knut Einar.
In: Electrophoresis, Vol. 19, No. 16-17, 01.11.1998, p. 2912-2917.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Analysis of vitamin formulations by electrokinetic chromatography utilizing tetradecylammonium ions as the pseudostationary phase
AU - Næss, Øystein
AU - Tilander, Tuija
AU - Pedersen-Bjergaard, Stig
AU - Rasmussen, Knut Einar
PY - 1998/11/1
Y1 - 1998/11/1
N2 - A recently proposed method for the separation of fat-soluble vitamins by electrokinetic chromatography was further developed and investigated in the present study. The separation medium consisted of acetonitrile-water (80:20 v/v) and contained 80 mM tetradecylammonium bromide (TDA+); the content of acetonitrile served to maintain the hydrophobic vitamins dissolved during electrophoresis, while the TDA+ ions served as the pseudostationary phase. With the cathode placed at the outlet of the capillary, the fat-soluble vitamins were separated based on different hydrophobic interactions to the TDA+ ions and migrated in order of decreasing hydrophobicity prior to the electroosmotic flow. Migration time stability was significantly enhanced by the addition of 4 mu borate to the separation medium. The separation system was validated for the determination of vitamin E acetate in commercial tablets; quantitative results deviated by less than 3.5% from specified values, varying by less than 2.5% relative standard deviation (RSD) for within-day experiments, and by less than 6.5% RSD during between-day experiments. The separation system was compatible with injection solvents ranging in polarity from water to tetrahydrofuran, and was even capable of separating the water-soluble vitamins B1, B2, B12, and nicotinamide.
AB - A recently proposed method for the separation of fat-soluble vitamins by electrokinetic chromatography was further developed and investigated in the present study. The separation medium consisted of acetonitrile-water (80:20 v/v) and contained 80 mM tetradecylammonium bromide (TDA+); the content of acetonitrile served to maintain the hydrophobic vitamins dissolved during electrophoresis, while the TDA+ ions served as the pseudostationary phase. With the cathode placed at the outlet of the capillary, the fat-soluble vitamins were separated based on different hydrophobic interactions to the TDA+ ions and migrated in order of decreasing hydrophobicity prior to the electroosmotic flow. Migration time stability was significantly enhanced by the addition of 4 mu borate to the separation medium. The separation system was validated for the determination of vitamin E acetate in commercial tablets; quantitative results deviated by less than 3.5% from specified values, varying by less than 2.5% relative standard deviation (RSD) for within-day experiments, and by less than 6.5% RSD during between-day experiments. The separation system was compatible with injection solvents ranging in polarity from water to tetrahydrofuran, and was even capable of separating the water-soluble vitamins B1, B2, B12, and nicotinamide.
KW - Electrokinetic chromatography
KW - Fat-soluble vitamins
KW - Pharmaceutical products
KW - Tetradecylammonium bromide
KW - Water-soluble vitamins
UR - http://www.scopus.com/inward/record.url?scp=0031798020&partnerID=8YFLogxK
M3 - Journal article
C2 - 9870388
AN - SCOPUS:0031798020
VL - 19
SP - 2912
EP - 2917
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 16-17
ER -
ID: 231655094