Mapping surface charge density of lipid bilayers by quantitative surface conductivity microscopy

Research output: Contribution to journal › Journal article › Research › peer-review

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Mapping surface charge density of lipid bilayers by quantitative surface conductivity microscopy. / Klausen, Lasse Hyldgaard; Fuhs, Thomas; Dong, Mingdong.

In: Nature Communications, Vol. 7, 12447, 26.08.2016, p. 1-10.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Klausen, LH, Fuhs, T & Dong, M 2016, 'Mapping surface charge density of lipid bilayers by quantitative surface conductivity microscopy', Nature Communications, vol. 7, 12447, pp. 1-10. https://doi.org/10.1038/ncomms12447

APA

Klausen, L. H., Fuhs, T., & Dong, M. (2016). Mapping surface charge density of lipid bilayers by quantitative surface conductivity microscopy. Nature Communications, 7, 1-10. [12447]. https://doi.org/10.1038/ncomms12447

Vancouver

Klausen LH, Fuhs T, Dong M. Mapping surface charge density of lipid bilayers by quantitative surface conductivity microscopy. Nature Communications. 2016 Aug 26;7:1-10. 12447. https://doi.org/10.1038/ncomms12447

Author

Klausen, Lasse Hyldgaard ; Fuhs, Thomas ; Dong, Mingdong. / Mapping surface charge density of lipid bilayers by quantitative surface conductivity microscopy. In: Nature Communications. 2016 ; Vol. 7. pp. 1-10.

Bibtex

@article{7ff5d11d86a34fa89ac038b17e5f7f29,
title = "Mapping surface charge density of lipid bilayers by quantitative surface conductivity microscopy",
abstract = "Local surface charge density of lipid membranes influences membrane-protein interactions leading to distinct functions in all living cells, and it is a vital parameter in understanding membrane-binding mechanisms, liposome design and drug delivery. Despite the significance, no method has so far been capable of mapping surface charge densities under physiologically relevant conditions. Here, we use a scanning nanopipette setup (scanning ion-conductance microscope) combined with a novel algorithm to investigate the surface conductivity near supported lipid bilayers, and we present a new approach, quantitative surface conductivity microscopy (QSCM), capable of mapping surface charge density with high-quantitative precision and nanoscale resolution. The method is validated through an extensive theoretical analysis of the ionic current at the nanopipette tip, and we demonstrate the capacity of QSCM by mapping the surface charge density of model cationic, anionic and zwitterionic lipids with results accurately matching theoretical values.",
keywords = "Journal Article",
author = "Klausen, {Lasse Hyldgaard} and Thomas Fuhs and Mingdong Dong",
year = "2016",
month = aug,
day = "26",
doi = "10.1038/ncomms12447",
language = "English",
volume = "7",
pages = "1--10",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Mapping surface charge density of lipid bilayers by quantitative surface conductivity microscopy

AU - Klausen, Lasse Hyldgaard

AU - Fuhs, Thomas

AU - Dong, Mingdong

PY - 2016/8/26

Y1 - 2016/8/26

N2 - Local surface charge density of lipid membranes influences membrane-protein interactions leading to distinct functions in all living cells, and it is a vital parameter in understanding membrane-binding mechanisms, liposome design and drug delivery. Despite the significance, no method has so far been capable of mapping surface charge densities under physiologically relevant conditions. Here, we use a scanning nanopipette setup (scanning ion-conductance microscope) combined with a novel algorithm to investigate the surface conductivity near supported lipid bilayers, and we present a new approach, quantitative surface conductivity microscopy (QSCM), capable of mapping surface charge density with high-quantitative precision and nanoscale resolution. The method is validated through an extensive theoretical analysis of the ionic current at the nanopipette tip, and we demonstrate the capacity of QSCM by mapping the surface charge density of model cationic, anionic and zwitterionic lipids with results accurately matching theoretical values.

AB - Local surface charge density of lipid membranes influences membrane-protein interactions leading to distinct functions in all living cells, and it is a vital parameter in understanding membrane-binding mechanisms, liposome design and drug delivery. Despite the significance, no method has so far been capable of mapping surface charge densities under physiologically relevant conditions. Here, we use a scanning nanopipette setup (scanning ion-conductance microscope) combined with a novel algorithm to investigate the surface conductivity near supported lipid bilayers, and we present a new approach, quantitative surface conductivity microscopy (QSCM), capable of mapping surface charge density with high-quantitative precision and nanoscale resolution. The method is validated through an extensive theoretical analysis of the ionic current at the nanopipette tip, and we demonstrate the capacity of QSCM by mapping the surface charge density of model cationic, anionic and zwitterionic lipids with results accurately matching theoretical values.

KW - Journal Article

U2 - 10.1038/ncomms12447

DO - 10.1038/ncomms12447

M3 - Journal article

C2 - 27561322

VL - 7

SP - 1

EP - 10

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 12447

ER -

ID: 169359018