Thioflavin T templates amyloid β(1-40) conformation and aggregation pathway
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Thioflavin T templates amyloid β(1-40) conformation and aggregation pathway. / Di Carlo, Maria Giovanna; Minicozzi, Velia; Foderà, Vito; Militello, Valeria; Vetri, Valeria; Morante, Silvia; Leone, Maurizio.
In: Biophysical Chemistry, Vol. 206, 11.2015, p. 1-11.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Thioflavin T templates amyloid β(1-40) conformation and aggregation pathway
AU - Di Carlo, Maria Giovanna
AU - Minicozzi, Velia
AU - Foderà, Vito
AU - Militello, Valeria
AU - Vetri, Valeria
AU - Morante, Silvia
AU - Leone, Maurizio
N1 - Copyright © 2015 Elsevier B.V. All rights reserved.
PY - 2015/11
Y1 - 2015/11
N2 - Aβ(1-40) peptide supramolecular assembly and fibril formation processes are widely recognized to have direct implications in the progression of Alzheimer's disease. The molecular basis of this biological process is still unknown and there is a strong need of developing effective strategies to control the occurring events. To this purpose the exploitation of small molecules interacting with Aβ aggregation represents one of the possible routes. Moreover, the use specific labeling has represented so far one of the most common and effective methods to investigate such a process. This possibility in turn rests on the reliability of the probe/labels involved. Here we present evidences of the effect of Thioflavin T (ThT), a worldwide used fluorescent dye to monitor amyloid growth, on the Aβ(1-40) conformation, stability and aggregation. By combining experimental information and Molecular Dynamics simulation results, we show that the presence of ThT in solution affects peptide conformation inducing peculiar supramolecular association. In particular ThT interactions with specific Aβ(1-40) residues promote a rigid partially-folded conformation which shifts the balance between different species in solution toward a more aggregation-prone ensemble of peptides, leading to aggregation. Our findings suggest ways for developing strategies to reverse and block aggregation or to stimulate supramolecular assembly and consequently reduce the presence of transient oligomers. This investigation underlines the need of developing label-free techniques for unbiased quantitative studies of Aβ(1-40) aggregation processes.
AB - Aβ(1-40) peptide supramolecular assembly and fibril formation processes are widely recognized to have direct implications in the progression of Alzheimer's disease. The molecular basis of this biological process is still unknown and there is a strong need of developing effective strategies to control the occurring events. To this purpose the exploitation of small molecules interacting with Aβ aggregation represents one of the possible routes. Moreover, the use specific labeling has represented so far one of the most common and effective methods to investigate such a process. This possibility in turn rests on the reliability of the probe/labels involved. Here we present evidences of the effect of Thioflavin T (ThT), a worldwide used fluorescent dye to monitor amyloid growth, on the Aβ(1-40) conformation, stability and aggregation. By combining experimental information and Molecular Dynamics simulation results, we show that the presence of ThT in solution affects peptide conformation inducing peculiar supramolecular association. In particular ThT interactions with specific Aβ(1-40) residues promote a rigid partially-folded conformation which shifts the balance between different species in solution toward a more aggregation-prone ensemble of peptides, leading to aggregation. Our findings suggest ways for developing strategies to reverse and block aggregation or to stimulate supramolecular assembly and consequently reduce the presence of transient oligomers. This investigation underlines the need of developing label-free techniques for unbiased quantitative studies of Aβ(1-40) aggregation processes.
KW - Alzheimer Disease
KW - Amino Acid Sequence
KW - Amyloid beta-Peptides
KW - Fluorescence Recovery After Photobleaching
KW - Fluorescent Dyes
KW - Humans
KW - Molecular Dynamics Simulation
KW - Molecular Sequence Data
KW - Peptide Fragments
KW - Protein Aggregates
KW - Protein Aggregation, Pathological
KW - Protein Conformation
KW - Protein Multimerization
KW - Protein Stability
KW - Protein Structure, Secondary
KW - Thiazoles
U2 - 10.1016/j.bpc.2015.06.006
DO - 10.1016/j.bpc.2015.06.006
M3 - Journal article
C2 - 26100600
VL - 206
SP - 1
EP - 11
JO - Biophysical Chemistry
JF - Biophysical Chemistry
SN - 0301-4622
ER -
ID: 161623979