Production of Intrinsically Disordered Proteins for Biophysical Studies: Tips and Tricks

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Production of Intrinsically Disordered Proteins for Biophysical Studies : Tips and Tricks. / Pedersen, Christian Parsbæk; Seiffert, Pernille; Brakti, Inna; Bugge, Katrine.

Intrinsically Disordered Proteins: Methods and Protocols. ed. / Birthe B. Kragelund; Karen Skriver. Humana Press, 2020. p. 195-209 (Methods in Molecular Biology, Vol. 2141).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Pedersen, CP, Seiffert, P, Brakti, I & Bugge, K 2020, Production of Intrinsically Disordered Proteins for Biophysical Studies: Tips and Tricks. in BB Kragelund & K Skriver (eds), Intrinsically Disordered Proteins: Methods and Protocols. Humana Press, Methods in Molecular Biology, vol. 2141, pp. 195-209. https://doi.org/10.1007/978-1-0716-0524-0_9

APA

Pedersen, C. P., Seiffert, P., Brakti, I., & Bugge, K. (2020). Production of Intrinsically Disordered Proteins for Biophysical Studies: Tips and Tricks. In B. B. Kragelund, & K. Skriver (Eds.), Intrinsically Disordered Proteins: Methods and Protocols (pp. 195-209). Humana Press. Methods in Molecular Biology Vol. 2141 https://doi.org/10.1007/978-1-0716-0524-0_9

Vancouver

Pedersen CP, Seiffert P, Brakti I, Bugge K. Production of Intrinsically Disordered Proteins for Biophysical Studies: Tips and Tricks. In Kragelund BB, Skriver K, editors, Intrinsically Disordered Proteins: Methods and Protocols. Humana Press. 2020. p. 195-209. (Methods in Molecular Biology, Vol. 2141). https://doi.org/10.1007/978-1-0716-0524-0_9

Author

Pedersen, Christian Parsbæk ; Seiffert, Pernille ; Brakti, Inna ; Bugge, Katrine. / Production of Intrinsically Disordered Proteins for Biophysical Studies : Tips and Tricks. Intrinsically Disordered Proteins: Methods and Protocols. editor / Birthe B. Kragelund ; Karen Skriver. Humana Press, 2020. pp. 195-209 (Methods in Molecular Biology, Vol. 2141).

Bibtex

@inbook{719070aec42b44119eb5d17849340205,
title = "Production of Intrinsically Disordered Proteins for Biophysical Studies: Tips and Tricks",
abstract = "Intrinsically disordered proteins (IDPs) have no single, fixed tertiary structure, yet they take on many vital functions in biology. In recent years, considerable effort has been put into the structural characterization of their conformational ensembles, to understand the link between the transient, short- and long-range organizations of IDPs and their functions. Such biophysical studies require substantial amounts of pure protein, representing a major bottleneck in the studies of IDPs. However, the unique physicochemical properties resulting from their compositional bias may be exploited for simple yet effective purification strategies. In this chapter, we provide tips and tricks for IDP production and describe the most important analyses to carry out before bringing an IDP of interest to the laboratory. We outline four purification protocols utilizing the unique properties of IDPs as well as some commonly encountered challenges and pitfalls.",
keywords = "Heat treatment, IDP, Intrinsically disordered proteins, Ion-exchange chromatography, Isoelectric precipitation, Protein purification, Purification optimization, Reversed-phase chromatography, Troubleshooting",
author = "Pedersen, {Christian Parsb{\ae}k} and Pernille Seiffert and Inna Brakti and Katrine Bugge",
year = "2020",
doi = "10.1007/978-1-0716-0524-0_9",
language = "English",
isbn = "978-1-0716-0523-3",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "195--209",
editor = "Kragelund, {Birthe B.} and Karen Skriver",
booktitle = "Intrinsically Disordered Proteins",
address = "United States",

}

RIS

TY - CHAP

T1 - Production of Intrinsically Disordered Proteins for Biophysical Studies

T2 - Tips and Tricks

AU - Pedersen, Christian Parsbæk

AU - Seiffert, Pernille

AU - Brakti, Inna

AU - Bugge, Katrine

PY - 2020

Y1 - 2020

N2 - Intrinsically disordered proteins (IDPs) have no single, fixed tertiary structure, yet they take on many vital functions in biology. In recent years, considerable effort has been put into the structural characterization of their conformational ensembles, to understand the link between the transient, short- and long-range organizations of IDPs and their functions. Such biophysical studies require substantial amounts of pure protein, representing a major bottleneck in the studies of IDPs. However, the unique physicochemical properties resulting from their compositional bias may be exploited for simple yet effective purification strategies. In this chapter, we provide tips and tricks for IDP production and describe the most important analyses to carry out before bringing an IDP of interest to the laboratory. We outline four purification protocols utilizing the unique properties of IDPs as well as some commonly encountered challenges and pitfalls.

AB - Intrinsically disordered proteins (IDPs) have no single, fixed tertiary structure, yet they take on many vital functions in biology. In recent years, considerable effort has been put into the structural characterization of their conformational ensembles, to understand the link between the transient, short- and long-range organizations of IDPs and their functions. Such biophysical studies require substantial amounts of pure protein, representing a major bottleneck in the studies of IDPs. However, the unique physicochemical properties resulting from their compositional bias may be exploited for simple yet effective purification strategies. In this chapter, we provide tips and tricks for IDP production and describe the most important analyses to carry out before bringing an IDP of interest to the laboratory. We outline four purification protocols utilizing the unique properties of IDPs as well as some commonly encountered challenges and pitfalls.

KW - Heat treatment

KW - IDP

KW - Intrinsically disordered proteins

KW - Ion-exchange chromatography

KW - Isoelectric precipitation

KW - Protein purification

KW - Purification optimization

KW - Reversed-phase chromatography

KW - Troubleshooting

U2 - 10.1007/978-1-0716-0524-0_9

DO - 10.1007/978-1-0716-0524-0_9

M3 - Book chapter

C2 - 32696358

AN - SCOPUS:85088499750

SN - 978-1-0716-0523-3

T3 - Methods in Molecular Biology

SP - 195

EP - 209

BT - Intrinsically Disordered Proteins

A2 - Kragelund, Birthe B.

A2 - Skriver, Karen

PB - Humana Press

ER -

ID: 247383768