TY - JOUR

T1 - Peptide-Membrane Interactions Monitored by Fluorescence Lifetime Imaging

T2 - A Study Case of Transportan 10

AU - Anselmo, Sara

AU - Sancataldo, Giuseppe

AU - Mørck Nielsen, Hanne

AU - Foderà, Vito

AU - Vetri, Valeria

N1 - Funding Information: The authors thank the University of Palermo for financial support (FFR─PROMETA project). Part of microscopy was performed at the Advanced Technologies Network (ATeN) Center, University of Palermo. G.S. has received funding from PON AIM1809078-1. Publisher Copyright: ©

PY - 2021

Y1 - 2021

N2 - The interest on detailed analysis of peptide-membrane interactions is of great interest in both fundamental and applied sciences as these may relate to both functional and pathogenic events. Such interactions are highly dynamic and spatially heterogeneous, making the investigation of the associated phenomena highly complex. The specific properties of membranes and peptide structural details, together with environmental conditions, may determine different events at the membrane interface, which will drive the fate of the peptide-membrane system. Here, we use an experimental approach based on the combination of spectroscopy and fluorescence microscopy methods to characterize the interactions of the multifunctional amphiphilic peptide transportan 10 with model membranes. Our approach, based on the use of suitable fluorescence reporters, exploits the advantages of phasor plot analysis of fluorescence lifetime imaging microscopy measurements to highlight the molecular details of occurring membrane alterations in terms of rigidity and hydration. Simultaneously, it allows following dynamic events in real time without sample manipulation distinguishing, with high spatial resolution, whether the peptide is adsorbed to or inserted in the membrane.

AB - The interest on detailed analysis of peptide-membrane interactions is of great interest in both fundamental and applied sciences as these may relate to both functional and pathogenic events. Such interactions are highly dynamic and spatially heterogeneous, making the investigation of the associated phenomena highly complex. The specific properties of membranes and peptide structural details, together with environmental conditions, may determine different events at the membrane interface, which will drive the fate of the peptide-membrane system. Here, we use an experimental approach based on the combination of spectroscopy and fluorescence microscopy methods to characterize the interactions of the multifunctional amphiphilic peptide transportan 10 with model membranes. Our approach, based on the use of suitable fluorescence reporters, exploits the advantages of phasor plot analysis of fluorescence lifetime imaging microscopy measurements to highlight the molecular details of occurring membrane alterations in terms of rigidity and hydration. Simultaneously, it allows following dynamic events in real time without sample manipulation distinguishing, with high spatial resolution, whether the peptide is adsorbed to or inserted in the membrane.

U2 - 10.1021/acs.langmuir.1c02392

DO - 10.1021/acs.langmuir.1c02392

M3 - Journal article

C2 - 34714654

AN - SCOPUS:85118797590

VL - 37

SP - 13148

EP - 13159

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 44

ER -