Exploring the effect of protein secondary structure on the solid state and physical stability of protein-based amorphous solid dispersions
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Exploring the effect of protein secondary structure on the solid state and physical stability of protein-based amorphous solid dispersions. / Zhuo, Xuezhi; Ochner, Julia; Leng, Donglei; Foderà, Vito; Löbmann, Korbinian.
In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 198, 114274, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Exploring the effect of protein secondary structure on the solid state and physical stability of protein-based amorphous solid dispersions
AU - Zhuo, Xuezhi
AU - Ochner, Julia
AU - Leng, Donglei
AU - Foderà, Vito
AU - Löbmann, Korbinian
N1 - Publisher Copyright: © 2024
PY - 2024
Y1 - 2024
N2 - Amorphous solid dispersions (ASDs) using proteins as carriers have emerged as a promising strategy for stabilizing amorphous drug molecules. Proteins possess diverse three-dimensional structures that significantly influence their own properties and may also impact the properties of ASDs. We prepared β-lactoglobulin (BLG) with different contents of β-sheet and α-helical secondary structures by initially dissolving BLG in different mixed solvents, containing different ratios of water, methanol/ethanol, and acetic acid, followed by spray drying of the solutions. Our findings revealed that an increase in α-helical content resulted in a decrease in the glass transition temperature (Tg) of the protein. Subsequently, we utilized the corresponding mixed solvents to dissolve both BLG and the model drug celecoxib (CEL), allowing the preparation of ASDs containing either β-sheet-rich or α-helix/random coil-rich BLG. Using spray drying, we successfully developed BLG-based ASDs with drug loadings ranging from 10 wt% to 90 wt%. At drug loadings below 40 wt%, samples prepared using both methods exhibited single-phase ASDs. However, heterogeneous systems formed when the drug loading exceeded 40 wt%. At higher drug loadings, physical stability assessments demonstrated that the α-helix/random coil-rich BLG structure exerted a more pronounced stabilizing effect on the drug-rich phase compared to the β-sheet-rich BLG. Overall, our results highlight the importance of considering protein secondary structure in the design of ASDs.
AB - Amorphous solid dispersions (ASDs) using proteins as carriers have emerged as a promising strategy for stabilizing amorphous drug molecules. Proteins possess diverse three-dimensional structures that significantly influence their own properties and may also impact the properties of ASDs. We prepared β-lactoglobulin (BLG) with different contents of β-sheet and α-helical secondary structures by initially dissolving BLG in different mixed solvents, containing different ratios of water, methanol/ethanol, and acetic acid, followed by spray drying of the solutions. Our findings revealed that an increase in α-helical content resulted in a decrease in the glass transition temperature (Tg) of the protein. Subsequently, we utilized the corresponding mixed solvents to dissolve both BLG and the model drug celecoxib (CEL), allowing the preparation of ASDs containing either β-sheet-rich or α-helix/random coil-rich BLG. Using spray drying, we successfully developed BLG-based ASDs with drug loadings ranging from 10 wt% to 90 wt%. At drug loadings below 40 wt%, samples prepared using both methods exhibited single-phase ASDs. However, heterogeneous systems formed when the drug loading exceeded 40 wt%. At higher drug loadings, physical stability assessments demonstrated that the α-helix/random coil-rich BLG structure exerted a more pronounced stabilizing effect on the drug-rich phase compared to the β-sheet-rich BLG. Overall, our results highlight the importance of considering protein secondary structure in the design of ASDs.
KW - Amorphous solid dispersion
KW - Celecoxib
KW - Glass transition temperature
KW - Protein secondary structure
KW - Stability
KW - β-lactoglobulin
U2 - 10.1016/j.ejpb.2024.114274
DO - 10.1016/j.ejpb.2024.114274
M3 - Journal article
C2 - 38561067
AN - SCOPUS:85189680429
VL - 198
JO - European Journal of Pharmaceutics and Biopharmaceutics
JF - European Journal of Pharmaceutics and Biopharmaceutics
SN - 0939-6411
M1 - 114274
ER -
ID: 388944762