Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells
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Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells. / Stie, Mai Bay; Thoke, Henrik Seir; Issinger, Olaf Georg; Hochscherf, Jennifer; Guerra, Barbara; Olsen, Lars F.
In: Colloids and Surfaces B: Biointerfaces, Vol. 174, 01.02.2019, p. 216-223.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Delivery of proteins encapsulated in chitosan-tripolyphosphate nanoparticles to human skin melanoma cells
AU - Stie, Mai Bay
AU - Thoke, Henrik Seir
AU - Issinger, Olaf Georg
AU - Hochscherf, Jennifer
AU - Guerra, Barbara
AU - Olsen, Lars F.
PY - 2019/2/1
Y1 - 2019/2/1
N2 - We have successfully encapsulated two proteins, bovine serum albumin (BSA) and p53, in chitosan-tripolyphosphate (TPP) nanoparticles at various pH values from 5.5 to 6.5 and delivered the particles to human melanoma cells. The particles have diameters ranging from 180 nm to 280 nm and a zeta potential of +15 to + 40 mV. Cellular uptake of the particles by human skin melanoma cells was evaluated by: (i) fluorescence microscopy and (ii) gel electrophoresis showing that FITC-labeled BSA and p53 could be recovered in the soluble cell fraction after lysis of the cells. Our data also show that the highest cellular uptake takes place at the lowest pH as the particles have the highest positive charge under these conditions. The method we describe appears to be a general method for delivery of proteins to cells using chitosan-TPP nanoparticles as a drug delivery system, since structurally unrelated proteins such as BSA and p53 with different isoelectrical points can be encapsulated in the chitosan-TPP nanoparticles and be effectively internalized by the cells.
AB - We have successfully encapsulated two proteins, bovine serum albumin (BSA) and p53, in chitosan-tripolyphosphate (TPP) nanoparticles at various pH values from 5.5 to 6.5 and delivered the particles to human melanoma cells. The particles have diameters ranging from 180 nm to 280 nm and a zeta potential of +15 to + 40 mV. Cellular uptake of the particles by human skin melanoma cells was evaluated by: (i) fluorescence microscopy and (ii) gel electrophoresis showing that FITC-labeled BSA and p53 could be recovered in the soluble cell fraction after lysis of the cells. Our data also show that the highest cellular uptake takes place at the lowest pH as the particles have the highest positive charge under these conditions. The method we describe appears to be a general method for delivery of proteins to cells using chitosan-TPP nanoparticles as a drug delivery system, since structurally unrelated proteins such as BSA and p53 with different isoelectrical points can be encapsulated in the chitosan-TPP nanoparticles and be effectively internalized by the cells.
KW - Chitosan
KW - Nanoparticles
KW - Protein
KW - SK-mel 28
KW - p53
U2 - 10.1016/j.colsurfb.2018.11.005
DO - 10.1016/j.colsurfb.2018.11.005
M3 - Journal article
C2 - 30465996
VL - 174
SP - 216
EP - 223
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -
ID: 216311117