Unleashing the healing potential: Exploring next-generation regenerative protein nanoscaffolds for burn wound recovery
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Unleashing the healing potential : Exploring next-generation regenerative protein nanoscaffolds for burn wound recovery. / Si, Liangwei; Guo, Xiong; Bera, Hriday; Chen, Yang; Xiu, Fangfang; Liu, Peixin; Zhao, Chunwei; Abbasi, Yasir Faraz; Tang, Xing; Foderà, Vito; Cun, Dongmei; Yang, Mingshi.
In: Asian Journal of Pharmaceutical Sciences, Vol. 18, No. 6, 100856, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Unleashing the healing potential
T2 - Exploring next-generation regenerative protein nanoscaffolds for burn wound recovery
AU - Si, Liangwei
AU - Guo, Xiong
AU - Bera, Hriday
AU - Chen, Yang
AU - Xiu, Fangfang
AU - Liu, Peixin
AU - Zhao, Chunwei
AU - Abbasi, Yasir Faraz
AU - Tang, Xing
AU - Foderà, Vito
AU - Cun, Dongmei
AU - Yang, Mingshi
N1 - Publisher Copyright: © 2023
PY - 2023
Y1 - 2023
N2 - Burn injury is a serious public health problem and scientists are continuously aiming to develop promising biomimetic dressings for effective burn wound management. In this study, a greater efficacy in burn wound healing and the associated mechanisms of α-lactalbumin (ALA) based electrospun nanofibrous scaffolds (ENs) as compared to other regenerative protein scaffolds were established. Bovine serum albumin (BSA), collagen type I (COL), lysozyme (LZM) and ALA were separately blended with poly(ε-caprolactone) (PCL) to fabricate four different composite ENs (LZM/PCL, BSA/PCL, COL/PCL and ALA/PCL ENs). The hydrophilic composite scaffolds exhibited an enhanced wettability and variable mechanical properties. The ALA/PCL ENs demonstrated higher levels of fibroblast proliferation and adhesion than the other composite ENs. As compared to PCL ENs and other composite scaffolds, the ALA/PCL ENs also promoted a better maturity of the regenerative skin tissues and showed a comparable wound healing effect to Collagen spongeⓇ on third-degree burn model. The enhanced wound healing activity of ALA/PCL ENs compared to other ENs could be attributed to their ability to promote serotonin production at wound sites. Collectively, this investigation demonstrated that ALA is a unique protein with a greater potential for burn wound healing as compared to other regenerative proteins when loaded in the nanofibrous scaffolds.
AB - Burn injury is a serious public health problem and scientists are continuously aiming to develop promising biomimetic dressings for effective burn wound management. In this study, a greater efficacy in burn wound healing and the associated mechanisms of α-lactalbumin (ALA) based electrospun nanofibrous scaffolds (ENs) as compared to other regenerative protein scaffolds were established. Bovine serum albumin (BSA), collagen type I (COL), lysozyme (LZM) and ALA were separately blended with poly(ε-caprolactone) (PCL) to fabricate four different composite ENs (LZM/PCL, BSA/PCL, COL/PCL and ALA/PCL ENs). The hydrophilic composite scaffolds exhibited an enhanced wettability and variable mechanical properties. The ALA/PCL ENs demonstrated higher levels of fibroblast proliferation and adhesion than the other composite ENs. As compared to PCL ENs and other composite scaffolds, the ALA/PCL ENs also promoted a better maturity of the regenerative skin tissues and showed a comparable wound healing effect to Collagen spongeⓇ on third-degree burn model. The enhanced wound healing activity of ALA/PCL ENs compared to other ENs could be attributed to their ability to promote serotonin production at wound sites. Collectively, this investigation demonstrated that ALA is a unique protein with a greater potential for burn wound healing as compared to other regenerative proteins when loaded in the nanofibrous scaffolds.
KW - Electrospinning
KW - Nanofibrous dressing
KW - Regenerative proteins
KW - Serotonin
KW - Third-degree burn
KW - α-lactalbumin
U2 - 10.1016/j.ajps.2023.100856
DO - 10.1016/j.ajps.2023.100856
M3 - Journal article
C2 - 38204470
AN - SCOPUS:85178111682
VL - 18
JO - Asian Journal of Pharmaceutical Sciences
JF - Asian Journal of Pharmaceutical Sciences
SN - 1818-0876
IS - 6
M1 - 100856
ER -
ID: 375720297