Transcutaneous immunization using microneedles and cubosomes: Mechanistic investigations using Optical Coherence Tomography and Two-Photon Microscopy
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Transcutaneous immunization using microneedles and cubosomes : Mechanistic investigations using Optical Coherence Tomography and Two-Photon Microscopy. / Rattanapak, Teerawan; Birchall, James; Young, Katherine; Ishii, Masaru; Meglinski, Igor; Rades, Thomas; Hook, Sarah.
In: Journal of controlled release : official journal of the Controlled Release Society, Vol. 172, No. 3, 28.12.2013, p. 894-903.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Transcutaneous immunization using microneedles and cubosomes
T2 - Mechanistic investigations using Optical Coherence Tomography and Two-Photon Microscopy
AU - Rattanapak, Teerawan
AU - Birchall, James
AU - Young, Katherine
AU - Ishii, Masaru
AU - Meglinski, Igor
AU - Rades, Thomas
AU - Hook, Sarah
N1 - © 2013.
PY - 2013/12/28
Y1 - 2013/12/28
N2 - Transcutaneous (TCI) immunization is a novel vaccination approach that provides many advantages over traditional parenteral vaccination. However, a major barrier to TCI is mediating penetration of vaccine antigens through the stratum corneum (SC) to the deeper tissue layers. Many approaches have been investigated for enhancing drug penetration into the skin including microneedles (MNs) to transiently breach the SC barrier and incorporation of vaccines into penetration-enhancing delivery systems. This study utilized MNs and a lipid-based colloidal delivery system (cubosomes) as a synergistic approach for the delivery of vaccines through the skin. The penetration and permeation of the peptide antigen into and through skin were analysed quantitatively and qualitatively using techniques including Optical Coherence Tomography and two-photon microscopy. It was found that while the use of MNs increased the permeation of an aqueous peptide mixture through skin, cubosome-formulated peptide and cubosomes were retained in the skin. Therefore, it is proposed that a combined approach using MNs and cubosomes will be an efficient system for the local delivery of antigen to immunocompetent cells in the skin.
AB - Transcutaneous (TCI) immunization is a novel vaccination approach that provides many advantages over traditional parenteral vaccination. However, a major barrier to TCI is mediating penetration of vaccine antigens through the stratum corneum (SC) to the deeper tissue layers. Many approaches have been investigated for enhancing drug penetration into the skin including microneedles (MNs) to transiently breach the SC barrier and incorporation of vaccines into penetration-enhancing delivery systems. This study utilized MNs and a lipid-based colloidal delivery system (cubosomes) as a synergistic approach for the delivery of vaccines through the skin. The penetration and permeation of the peptide antigen into and through skin were analysed quantitatively and qualitatively using techniques including Optical Coherence Tomography and two-photon microscopy. It was found that while the use of MNs increased the permeation of an aqueous peptide mixture through skin, cubosome-formulated peptide and cubosomes were retained in the skin. Therefore, it is proposed that a combined approach using MNs and cubosomes will be an efficient system for the local delivery of antigen to immunocompetent cells in the skin.
U2 - 10.1016/j.jconrel.2013.08.018
DO - 10.1016/j.jconrel.2013.08.018
M3 - Journal article
C2 - 23978683
VL - 172
SP - 894
EP - 903
JO - Journal of Controlled Release
JF - Journal of Controlled Release
SN - 0168-3659
IS - 3
ER -
ID: 104835011