Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses. / Jönsson, Alexander; Svejdal, Rasmus R; Bøgelund, Nanna; Nguyen, Tam T T N; Flindt, Henrik; Kutter, Jörg P; Rand, Kasper D; Lafleur, Josiane P.

In: Analytical Chemistry, Vol. 89, No. 8, 21.03.2017, p. 4573-4580.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jönsson, A, Svejdal, RR, Bøgelund, N, Nguyen, TTTN, Flindt, H, Kutter, JP, Rand, KD & Lafleur, JP 2017, 'Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses', Analytical Chemistry, vol. 89, no. 8, pp. 4573-4580. https://doi.org/10.1021/acs.analchem.6b05103

APA

Jönsson, A., Svejdal, R. R., Bøgelund, N., Nguyen, T. T. T. N., Flindt, H., Kutter, J. P., Rand, K. D., & Lafleur, J. P. (2017). Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses. Analytical Chemistry, 89(8), 4573-4580. https://doi.org/10.1021/acs.analchem.6b05103

Vancouver

Jönsson A, Svejdal RR, Bøgelund N, Nguyen TTTN, Flindt H, Kutter JP et al. Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses. Analytical Chemistry. 2017 Mar 21;89(8):4573-4580. https://doi.org/10.1021/acs.analchem.6b05103

Author

Jönsson, Alexander ; Svejdal, Rasmus R ; Bøgelund, Nanna ; Nguyen, Tam T T N ; Flindt, Henrik ; Kutter, Jörg P ; Rand, Kasper D ; Lafleur, Josiane P. / Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses. In: Analytical Chemistry. 2017 ; Vol. 89, No. 8. pp. 4573-4580.

Bibtex

@article{f9972df2c07544109d6e0723b28610d2,
title = "Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses",
abstract = "To improve the sample handling, and reduce cost and preparation time, of peptide mapping LC-MS workflows in protein analytical research, we here investigate the possibility of replacing conventional enzymatic digestion methods with a polymer microfluidic chip based enzyme reactor. Off-stoichiometric thiol-ene is utilized as both bulk material and as a monolithic stationary phase for immobilization of the proteolytic enzyme pepsin. The digestion efficiency of the, thiol-ene based, immobilized enzyme reactor (IMER) is compared to that of a conventional, agarose packed bed, pepsin IMER column commonly used in LC-MS based protein analyses. The chip IMER is found to rival the conventional column in terms of digestion efficiency at comparable residence time and, using a 3D-printed interface, be directly interfaceable with LC-MS.",
keywords = "Journal Article",
author = "Alexander J{\"o}nsson and Svejdal, {Rasmus R} and Nanna B{\o}gelund and Nguyen, {Tam T T N} and Henrik Flindt and Kutter, {J{\"o}rg P} and Rand, {Kasper D} and Lafleur, {Josiane P}",
year = "2017",
month = mar,
day = "21",
doi = "10.1021/acs.analchem.6b05103",
language = "English",
volume = "89",
pages = "4573--4580",
journal = "Industrial And Engineering Chemistry Analytical Edition",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "8",

}

RIS

TY - JOUR

T1 - Thiol-ene Monolithic Pepsin Microreactor with a 3D-Printed Interface for Efficient UPLC-MS Peptide Mapping Analyses

AU - Jönsson, Alexander

AU - Svejdal, Rasmus R

AU - Bøgelund, Nanna

AU - Nguyen, Tam T T N

AU - Flindt, Henrik

AU - Kutter, Jörg P

AU - Rand, Kasper D

AU - Lafleur, Josiane P

PY - 2017/3/21

Y1 - 2017/3/21

N2 - To improve the sample handling, and reduce cost and preparation time, of peptide mapping LC-MS workflows in protein analytical research, we here investigate the possibility of replacing conventional enzymatic digestion methods with a polymer microfluidic chip based enzyme reactor. Off-stoichiometric thiol-ene is utilized as both bulk material and as a monolithic stationary phase for immobilization of the proteolytic enzyme pepsin. The digestion efficiency of the, thiol-ene based, immobilized enzyme reactor (IMER) is compared to that of a conventional, agarose packed bed, pepsin IMER column commonly used in LC-MS based protein analyses. The chip IMER is found to rival the conventional column in terms of digestion efficiency at comparable residence time and, using a 3D-printed interface, be directly interfaceable with LC-MS.

AB - To improve the sample handling, and reduce cost and preparation time, of peptide mapping LC-MS workflows in protein analytical research, we here investigate the possibility of replacing conventional enzymatic digestion methods with a polymer microfluidic chip based enzyme reactor. Off-stoichiometric thiol-ene is utilized as both bulk material and as a monolithic stationary phase for immobilization of the proteolytic enzyme pepsin. The digestion efficiency of the, thiol-ene based, immobilized enzyme reactor (IMER) is compared to that of a conventional, agarose packed bed, pepsin IMER column commonly used in LC-MS based protein analyses. The chip IMER is found to rival the conventional column in terms of digestion efficiency at comparable residence time and, using a 3D-printed interface, be directly interfaceable with LC-MS.

KW - Journal Article

U2 - 10.1021/acs.analchem.6b05103

DO - 10.1021/acs.analchem.6b05103

M3 - Journal article

C2 - 28322047

VL - 89

SP - 4573

EP - 4580

JO - Industrial And Engineering Chemistry Analytical Edition

JF - Industrial And Engineering Chemistry Analytical Edition

SN - 0003-2700

IS - 8

ER -

ID: 176596936