SPECT/CT imaging of radiolabeled cubosomes and hexosomes for potential theranostic applications
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SPECT/CT imaging of radiolabeled cubosomes and hexosomes for potential theranostic applications. / Nilsson, Christa; Barrios-Lopez, Brianda; Kallinen, Annukka; Laurinmäki, Pasi; Butcher, Sarah J; Raki, Mari; Weisell, Janne; Bergström, Kim; Larsen, Susan Weng; Ostergaard, Jesper; Larsen, Claus; Urtti, Arto; Airaksinen, Anu J; Yaghmur, Anan.
In: Biomaterials, Vol. 34, No. 33, 07.08.2013, p. 8491-8503.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - SPECT/CT imaging of radiolabeled cubosomes and hexosomes for potential theranostic applications
AU - Nilsson, Christa
AU - Barrios-Lopez, Brianda
AU - Kallinen, Annukka
AU - Laurinmäki, Pasi
AU - Butcher, Sarah J
AU - Raki, Mari
AU - Weisell, Janne
AU - Bergström, Kim
AU - Larsen, Susan Weng
AU - Ostergaard, Jesper
AU - Larsen, Claus
AU - Urtti, Arto
AU - Airaksinen, Anu J
AU - Yaghmur, Anan
N1 - © 2013 Elsevier Ltd. All rights reserved.
PY - 2013/8/7
Y1 - 2013/8/7
N2 - We have developed a highly efficient method for the radiolabeling of phytantriol (PHYT)/oleic acid (OA)-based hexosomes based on the surface chelation of technetium-99m ((99m)Tc) to preformed hexosomes using the polyamine 1, 12-diamino-3, 6, 9-triazododecane (SpmTrien) as chelating agent. We also report on the unsuccessful labeling of cubosomes using the well-known chelating agent hexamethylpropyleneamine oxime (HMPAO). The (99m)Tc-labeled SpmTrien-hexosomes ((99m)Tc-SpmTrien-hexosomes) were synthesized with good radiolabeling (84%) and high radiochemical purity (>90%). The effect of radiolabeling on the internal nanostructure and the overall size of these aqueous dispersions was investigated by using synchrotron small angle X-ray scattering (SAXS), dynamic light scattering (DLS), and transmission electron cryo microscopy (cryo-TEM). Further, we show the utility of (99m)Tc-SpmTrien-hexosomes for the in vivo imaging of healthy mice using single photon emission computed tomography (SPECT) in combination with computed tomography (CT), i.e. SPECT/CT. SPECT/CT experiments of subcutaneously administered (99m)Tc-SpmTrien-hexosomes to the flank of mice showed a high stability in vivo allowing imaging of the distribution of the radiolabeled hexosomes for up to 24 h. These injected (99m)Tc-SpmTrien-hexosomes formed a deposit within the subcutaneous adipose tissue, displaying a high biodistribution of ∼343% injected dose/g tissue (%ID/g), with negligible uptake in other organs and tissues. The developed (99m)Tc labeling method for PHYT/OA-based hexosomes could further serve as a useful tool for investigating and imaging the in vivo performance of cubosomal and hexosomal drug nanocarriers.
AB - We have developed a highly efficient method for the radiolabeling of phytantriol (PHYT)/oleic acid (OA)-based hexosomes based on the surface chelation of technetium-99m ((99m)Tc) to preformed hexosomes using the polyamine 1, 12-diamino-3, 6, 9-triazododecane (SpmTrien) as chelating agent. We also report on the unsuccessful labeling of cubosomes using the well-known chelating agent hexamethylpropyleneamine oxime (HMPAO). The (99m)Tc-labeled SpmTrien-hexosomes ((99m)Tc-SpmTrien-hexosomes) were synthesized with good radiolabeling (84%) and high radiochemical purity (>90%). The effect of radiolabeling on the internal nanostructure and the overall size of these aqueous dispersions was investigated by using synchrotron small angle X-ray scattering (SAXS), dynamic light scattering (DLS), and transmission electron cryo microscopy (cryo-TEM). Further, we show the utility of (99m)Tc-SpmTrien-hexosomes for the in vivo imaging of healthy mice using single photon emission computed tomography (SPECT) in combination with computed tomography (CT), i.e. SPECT/CT. SPECT/CT experiments of subcutaneously administered (99m)Tc-SpmTrien-hexosomes to the flank of mice showed a high stability in vivo allowing imaging of the distribution of the radiolabeled hexosomes for up to 24 h. These injected (99m)Tc-SpmTrien-hexosomes formed a deposit within the subcutaneous adipose tissue, displaying a high biodistribution of ∼343% injected dose/g tissue (%ID/g), with negligible uptake in other organs and tissues. The developed (99m)Tc labeling method for PHYT/OA-based hexosomes could further serve as a useful tool for investigating and imaging the in vivo performance of cubosomal and hexosomal drug nanocarriers.
U2 - 10.1016/j.biomaterials.2013.07.055
DO - 10.1016/j.biomaterials.2013.07.055
M3 - Journal article
C2 - 23932247
VL - 34
SP - 8491
EP - 8503
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 33
ER -
ID: 49142021