This paper demonstrates the fabrication and performance of a capillary electrophoresis (CE) chip with a UV-assisted roll-to-plate fabrication mechanism. Patterning and lamination of the chip can be performed with fabrication speeds of up to 19 m/min (ca. 20 chips/min), offering a rapid and simple production method for medium to high volume chip fabrication. The performance of the CE chip is demonstrated with an electrophoretic separation of two different fluorescent dyes.