Review of structural design guiding the development of lipid nanoparticles for nucleic acid delivery
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- 1-s2.0-S1359029423000304-main
Final published version, 2.58 MB, PDF document
Lipid nanoparticles (LNPs) are the most versatile and successful gene delivery systems, notably highlighted by their use in vaccines against COVID-19. LNPs have a well-defined core–shell structure, each region with its own distinctive compositions, suited for a wide range of in vivo delivery applications. Here, we discuss how a detailed knowledge of LNP structure can guide LNP formulation to improve the efficiency of delivery of their nucleic acid payload. Perspectives are detailed on how LNP structural design can guide more efficient nucleic acid transfection. Views on key physical characterization techniques needed for such developments are outlined including opinions on biophysical approaches both correlating structure with functionality in biological fluids and improving their ability to escape the endosome and deliver they payload.
Original language | English |
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Article number | 101705 |
Journal | Current Opinion in Colloid and Interface Science |
Volume | 66 |
Number of pages | 16 |
ISSN | 1359-0294 |
DOIs | |
Publication status | Published - Aug 2023 |
Externally published | Yes |
Bibliographical note
Funding Information:
M.Y.A. would like to acknowledge Lennart Lindfors for his guidance in the LNP development field as well as reviewing this manuscript. M.C. thanks the Swedish Research Council grant numbers 2018-03990 , 2018-04833 , 2022-03322 and the IKUR Strategy under the collaboration agreement between Ikerbasque Foundation and Biofisika Bizkaia Fundazioa (FBB) on behalf of the Department of Education of the Basque Government. F.S. thanks Politecnico di Milano for funding through MSCA Seal of Excellence fellowship.
Publisher Copyright:
© 2023
- Cationic ionizable lipids, Lipid nanoparticles, Nucleic acid delicery, Structure-function
Research areas
ID: 380294086