Recovery, enrichment and selectivity in liquid-phase microextraction: Comparison with conventional liquid-liquid extraction
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Recovery, enrichment and selectivity in liquid-phase microextraction : Comparison with conventional liquid-liquid extraction. / Ho, Tung Si; Pedersen-Bjergaard, Stig; Rasmussen, Knut Einar.
In: Journal of Chromatography A, Vol. 963, No. 1-2, 19.07.2002, p. 3-17.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Recovery, enrichment and selectivity in liquid-phase microextraction
T2 - Comparison with conventional liquid-liquid extraction
AU - Ho, Tung Si
AU - Pedersen-Bjergaard, Stig
AU - Rasmussen, Knut Einar
PY - 2002/7/19
Y1 - 2002/7/19
N2 - Mathematical descriptions for extraction recovery and enrichment were applied for liquid-phase microextraction (LPME) and comparison with conventional two- and three-phase liquid-liquid extraction techniques (LLE) was made. The LPME theoretical calculations were verified by experimental determination of actual partition coefficients and by data obtained with LPME in a robust hollow fibre formate. With hollow fibre LPME operated in the two-phase mode, analytes were extracted from 1 to 4 ml aqueous samples into 25-50 μl of an organic solvent present in the pores and in the lumen of the porous hollow fibres. Compared with conventional two-phase LLE, two-phase LPME provided substantially higher enrichments for compounds with relatively large partition coefficients (Korg/d>500). In contrast, because of the large volume of organic solvent relative to the sample volume, LLE provided high recovery and moderate enrichment even for compounds with relatively low partition coefficients (Korg/d>5). Thus, two-phase LPME may be used for substantially enhanced extraction selectivity and enrichment of relatively hydrophobic analytes as compared with LLE whereas conventional two-phase LLE is superior for more hydrophilic analytes. Similar results were found for three-phase LPME where analytes where extracted from 1 to 4 ml aqueous samples through approximately 20 μl organic solvent immobilized within the pores of the hollow fibre and into 25 μl of an aqueous acceptor solution inside the lumen of the hollow fibre. The fundamental differences of LPME and LLE were further demonstrated with practical experiments on extraction of the basic drugs promethazine, methadone, and haloperidol from human plasma and urine.
AB - Mathematical descriptions for extraction recovery and enrichment were applied for liquid-phase microextraction (LPME) and comparison with conventional two- and three-phase liquid-liquid extraction techniques (LLE) was made. The LPME theoretical calculations were verified by experimental determination of actual partition coefficients and by data obtained with LPME in a robust hollow fibre formate. With hollow fibre LPME operated in the two-phase mode, analytes were extracted from 1 to 4 ml aqueous samples into 25-50 μl of an organic solvent present in the pores and in the lumen of the porous hollow fibres. Compared with conventional two-phase LLE, two-phase LPME provided substantially higher enrichments for compounds with relatively large partition coefficients (Korg/d>500). In contrast, because of the large volume of organic solvent relative to the sample volume, LLE provided high recovery and moderate enrichment even for compounds with relatively low partition coefficients (Korg/d>5). Thus, two-phase LPME may be used for substantially enhanced extraction selectivity and enrichment of relatively hydrophobic analytes as compared with LLE whereas conventional two-phase LLE is superior for more hydrophilic analytes. Similar results were found for three-phase LPME where analytes where extracted from 1 to 4 ml aqueous samples through approximately 20 μl organic solvent immobilized within the pores of the hollow fibre and into 25 μl of an aqueous acceptor solution inside the lumen of the hollow fibre. The fundamental differences of LPME and LLE were further demonstrated with practical experiments on extraction of the basic drugs promethazine, methadone, and haloperidol from human plasma and urine.
KW - Extraction methods
KW - Haloperidol
KW - Hollow fibres
KW - Liquid-liquid extraction
KW - Liquid-phase microextraction
KW - Mathematical modelling
KW - Methadone
KW - Partition coefficients
KW - Promethazine
KW - Selectivity
UR - http://www.scopus.com/inward/record.url?scp=0037135065&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(02)00215-7
DO - 10.1016/S0021-9673(02)00215-7
M3 - Journal article
C2 - 12187984
AN - SCOPUS:0037135065
VL - 963
SP - 3
EP - 17
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0301-4770
IS - 1-2
ER -
ID: 231652870