Rapid and simple preparation of thiol-ene emulsion-templated monoliths and their application as enzymatic microreactors
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Rapid and simple preparation of thiol-ene emulsion-templated monoliths and their application as enzymatic microreactors. / Lafleur, Josiane P; Senkbeil, Silja; Novotny, Jakub; Nys, Gwenaël; Bøgelund, Nanna; Rand, Kasper D; Foret, Frantisek; Kutter, Jörg P.
In: Lab On a Chip, Vol. 15, No. 10, 2015, p. 2162-72.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Rapid and simple preparation of thiol-ene emulsion-templated monoliths and their application as enzymatic microreactors
AU - Lafleur, Josiane P
AU - Senkbeil, Silja
AU - Novotny, Jakub
AU - Nys, Gwenaël
AU - Bøgelund, Nanna
AU - Rand, Kasper D
AU - Foret, Frantisek
AU - Kutter, Jörg P
PY - 2015
Y1 - 2015
N2 - A novel, rapid and simple method for the preparation of emulsion-templated monoliths in microfluidic channels based on thiol-ene chemistry is presented. The method allows monolith synthesis and anchoring inside thiol-ene microchannels in a single photoinitiated step. Characterization by scanning electron microscopy showed that the methanol-based emulsion templating process resulted in a network of highly interconnected and regular thiol-ene beads anchored solidly inside thiol-ene microchannels. Surface area measurements indicate that the monoliths are macroporous, with no or little micro- or mesopores. As a demonstration, galactose oxidase and peptide-N-glycosidase F (PNGase F) were immobilized at the surface of the synthesized thiol-ene monoliths via two different mechanisms. First, cysteine groups on the protein surface were used for reversible covalent linkage to free thiol functional groups on the monoliths. Second, covalent linkage was achieved via free primary amino groups on the protein surface by means of thiol-ene click chemistry and l-ascorbic acid linkage. Thus prepared galactose oxidase and PNGase F microreactors demonstrated good enzymatic activity in a galactose assay and the deglycosilation of ribonuclease B, respectively.
AB - A novel, rapid and simple method for the preparation of emulsion-templated monoliths in microfluidic channels based on thiol-ene chemistry is presented. The method allows monolith synthesis and anchoring inside thiol-ene microchannels in a single photoinitiated step. Characterization by scanning electron microscopy showed that the methanol-based emulsion templating process resulted in a network of highly interconnected and regular thiol-ene beads anchored solidly inside thiol-ene microchannels. Surface area measurements indicate that the monoliths are macroporous, with no or little micro- or mesopores. As a demonstration, galactose oxidase and peptide-N-glycosidase F (PNGase F) were immobilized at the surface of the synthesized thiol-ene monoliths via two different mechanisms. First, cysteine groups on the protein surface were used for reversible covalent linkage to free thiol functional groups on the monoliths. Second, covalent linkage was achieved via free primary amino groups on the protein surface by means of thiol-ene click chemistry and l-ascorbic acid linkage. Thus prepared galactose oxidase and PNGase F microreactors demonstrated good enzymatic activity in a galactose assay and the deglycosilation of ribonuclease B, respectively.
U2 - 10.1039/c5lc00224a
DO - 10.1039/c5lc00224a
M3 - Journal article
C2 - 25850955
VL - 15
SP - 2162
EP - 2172
JO - Lab on a Chip
JF - Lab on a Chip
SN - 1473-0197
IS - 10
ER -
ID: 137374227