Protein adsorption to poly(tetrafluoroethylene) membranes modified with grafted poly(acrylic acid) chains
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Protein adsorption to poly(tetrafluoroethylene) membranes modified with grafted poly(acrylic acid) chains. / Mohd Hidzir, Norsyahidah; Anitha, A.; Kepa, Katarzyna; Hill, David J. T.; Jorgensen, Lene; Grondahl, Lisbeth.
In: Biointerphases, Vol. 15, No. 3, 031011, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Protein adsorption to poly(tetrafluoroethylene) membranes modified with grafted poly(acrylic acid) chains
AU - Mohd Hidzir, Norsyahidah
AU - Anitha, A.
AU - Kepa, Katarzyna
AU - Hill, David J. T.
AU - Jorgensen, Lene
AU - Grondahl, Lisbeth
PY - 2020
Y1 - 2020
N2 - Protein adsorption to biomaterial surfaces is important for the function of such materials with anchorage-dependent cell adhesion requiring the presence of adsorbed proteins. The current study evaluated five solid surfaces with poly(acrylic acid) (PAA) grafted from the surface of a poly(tetrafluoroethylene) membrane with respect to the adsorption of serum albumin (SA), lactoferrin (Lf), and lysozyme (Lys) from a phosphate buffer and NaCl solution or water for specific combinations. With the use of x-ray photoelectron spectroscopy, the relative amounts and protein layer thickness were evaluated. SA adsorption was governed by ionic repulsive forces and hydrophobic interactions as evidenced from an increase in the protein adsorption at lowerpH (6.5 compared to 7.4) and a correlation with surface coverage when water (pH 6.5) was used as the medium. The adsorption of Lf and Lys followed similar trends for all samples. In general, ionic attractive forces dominated and a strong correlation of increasing protein adsorption with the PAA chain length was evident. This study concluded that all surfaces appear suitable for use in biomaterial applications where tissue ingrowth is desired and that the enhanced protein adsorption in a medium with high ionic strength (e.g., biological fluid) correlates with the PAA chain length rather than the surface coverage.
AB - Protein adsorption to biomaterial surfaces is important for the function of such materials with anchorage-dependent cell adhesion requiring the presence of adsorbed proteins. The current study evaluated five solid surfaces with poly(acrylic acid) (PAA) grafted from the surface of a poly(tetrafluoroethylene) membrane with respect to the adsorption of serum albumin (SA), lactoferrin (Lf), and lysozyme (Lys) from a phosphate buffer and NaCl solution or water for specific combinations. With the use of x-ray photoelectron spectroscopy, the relative amounts and protein layer thickness were evaluated. SA adsorption was governed by ionic repulsive forces and hydrophobic interactions as evidenced from an increase in the protein adsorption at lowerpH (6.5 compared to 7.4) and a correlation with surface coverage when water (pH 6.5) was used as the medium. The adsorption of Lf and Lys followed similar trends for all samples. In general, ionic attractive forces dominated and a strong correlation of increasing protein adsorption with the PAA chain length was evident. This study concluded that all surfaces appear suitable for use in biomaterial applications where tissue ingrowth is desired and that the enhanced protein adsorption in a medium with high ionic strength (e.g., biological fluid) correlates with the PAA chain length rather than the surface coverage.
KW - BOVINE SERUM-ALBUMIN
KW - POLYACRYLIC-ACID
KW - ACRYLIC-ACID
KW - AQUEOUS-SOLUTION
KW - RESPONSIVE BEHAVIOR
KW - ADSORBED PROTEINS
KW - HYDROGELS
KW - PH
KW - LYSOZYME
KW - BRUSHES
U2 - 10.1116/6.0000137
DO - 10.1116/6.0000137
M3 - Journal article
C2 - 32527100
VL - 15
JO - Biointerphases
JF - Biointerphases
SN - 1559-4106
IS - 3
M1 - 031011
ER -
ID: 247543436