Preparation and characterization of melittin-loaded poly (DL-lactic acid) or poly (DL-lactic-co-glycolic acid) microspheres made by the double emulsion method

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Preparation and characterization of melittin-loaded poly (DL-lactic acid) or poly (DL-lactic-co-glycolic acid) microspheres made by the double emulsion method. / Cui, Fude; Cun, Dongmei; Tao, Anjin; Yang, Mingshi; Shi, Kai; Zhao, Min; Guan, Ying.

In: Journal of Controlled Release, Vol. 107, No. 2, 2005, p. 310-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Cui, F, Cun, D, Tao, A, Yang, M, Shi, K, Zhao, M & Guan, Y 2005, 'Preparation and characterization of melittin-loaded poly (DL-lactic acid) or poly (DL-lactic-co-glycolic acid) microspheres made by the double emulsion method', Journal of Controlled Release, vol. 107, no. 2, pp. 310-9.

APA

Cui, F., Cun, D., Tao, A., Yang, M., Shi, K., Zhao, M., & Guan, Y. (2005). Preparation and characterization of melittin-loaded poly (DL-lactic acid) or poly (DL-lactic-co-glycolic acid) microspheres made by the double emulsion method. Journal of Controlled Release, 107(2), 310-9.

Vancouver

Cui F, Cun D, Tao A, Yang M, Shi K, Zhao M et al. Preparation and characterization of melittin-loaded poly (DL-lactic acid) or poly (DL-lactic-co-glycolic acid) microspheres made by the double emulsion method. Journal of Controlled Release. 2005;107(2):310-9.

Author

Cui, Fude ; Cun, Dongmei ; Tao, Anjin ; Yang, Mingshi ; Shi, Kai ; Zhao, Min ; Guan, Ying. / Preparation and characterization of melittin-loaded poly (DL-lactic acid) or poly (DL-lactic-co-glycolic acid) microspheres made by the double emulsion method. In: Journal of Controlled Release. 2005 ; Vol. 107, No. 2. pp. 310-9.

Bibtex

@article{fe62f31da4da4f9cba57644b212f5f62,
title = "Preparation and characterization of melittin-loaded poly (DL-lactic acid) or poly (DL-lactic-co-glycolic acid) microspheres made by the double emulsion method",
abstract = "The water soluble peptide, melittin, isolated from bee venom and composed of twenty-six amino acids, was encapsulated in poly (DL-lactic acid, PLA) and poly (DL-lactic-co-glycolic acid, PLGA) microspheres prepared by a multiple emulsion [(W1/O)W2] solvent evaporation method. The aim of this work was to develop a controlled release injection that would deliver the melittin over a period of about one month. The influence of various preparation parameters, such as the type of polymer, its concentration, stabilizer PVA concentration, volume of internal water phase and level of drug loading on the characteristics of the microspheres and drug release was investigated. It was found that the microspheres of about 5 microm in size can be produced in high encapsulation (up to 90%), and the melittin content in the microspheres was up to 10% (w/w). The drug release profiles in vitro exhibited a significant burst release, followed by a lag phase of little or no release and then a phase of constant melittin release. The type of polymer used was a critical factor in controlling the release of melittin from the microspheres. In this study, the rate of peptide release from the microspheres correlated well with the rate of polymer degradation. Moreover, melittin was released completely during the study period of 30 days, which agreed well with the polymer degradation rate.",
keywords = "Drug Carriers, Drug Compounding, Emulsions, Lactic Acid, Melitten, Microspheres, Particle Size, Polyglycolic Acid, Polymers, Polyvinyl Alcohol, Surface Properties",
author = "Fude Cui and Dongmei Cun and Anjin Tao and Mingshi Yang and Kai Shi and Min Zhao and Ying Guan",
year = "2005",
language = "English",
volume = "107",
pages = "310--9",
journal = "Journal of Controlled Release",
issn = "0168-3659",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Preparation and characterization of melittin-loaded poly (DL-lactic acid) or poly (DL-lactic-co-glycolic acid) microspheres made by the double emulsion method

AU - Cui, Fude

AU - Cun, Dongmei

AU - Tao, Anjin

AU - Yang, Mingshi

AU - Shi, Kai

AU - Zhao, Min

AU - Guan, Ying

PY - 2005

Y1 - 2005

N2 - The water soluble peptide, melittin, isolated from bee venom and composed of twenty-six amino acids, was encapsulated in poly (DL-lactic acid, PLA) and poly (DL-lactic-co-glycolic acid, PLGA) microspheres prepared by a multiple emulsion [(W1/O)W2] solvent evaporation method. The aim of this work was to develop a controlled release injection that would deliver the melittin over a period of about one month. The influence of various preparation parameters, such as the type of polymer, its concentration, stabilizer PVA concentration, volume of internal water phase and level of drug loading on the characteristics of the microspheres and drug release was investigated. It was found that the microspheres of about 5 microm in size can be produced in high encapsulation (up to 90%), and the melittin content in the microspheres was up to 10% (w/w). The drug release profiles in vitro exhibited a significant burst release, followed by a lag phase of little or no release and then a phase of constant melittin release. The type of polymer used was a critical factor in controlling the release of melittin from the microspheres. In this study, the rate of peptide release from the microspheres correlated well with the rate of polymer degradation. Moreover, melittin was released completely during the study period of 30 days, which agreed well with the polymer degradation rate.

AB - The water soluble peptide, melittin, isolated from bee venom and composed of twenty-six amino acids, was encapsulated in poly (DL-lactic acid, PLA) and poly (DL-lactic-co-glycolic acid, PLGA) microspheres prepared by a multiple emulsion [(W1/O)W2] solvent evaporation method. The aim of this work was to develop a controlled release injection that would deliver the melittin over a period of about one month. The influence of various preparation parameters, such as the type of polymer, its concentration, stabilizer PVA concentration, volume of internal water phase and level of drug loading on the characteristics of the microspheres and drug release was investigated. It was found that the microspheres of about 5 microm in size can be produced in high encapsulation (up to 90%), and the melittin content in the microspheres was up to 10% (w/w). The drug release profiles in vitro exhibited a significant burst release, followed by a lag phase of little or no release and then a phase of constant melittin release. The type of polymer used was a critical factor in controlling the release of melittin from the microspheres. In this study, the rate of peptide release from the microspheres correlated well with the rate of polymer degradation. Moreover, melittin was released completely during the study period of 30 days, which agreed well with the polymer degradation rate.

KW - Drug Carriers

KW - Drug Compounding

KW - Emulsions

KW - Lactic Acid

KW - Melitten

KW - Microspheres

KW - Particle Size

KW - Polyglycolic Acid

KW - Polymers

KW - Polyvinyl Alcohol

KW - Surface Properties

M3 - Journal article

C2 - 16255081

VL - 107

SP - 310

EP - 319

JO - Journal of Controlled Release

JF - Journal of Controlled Release

SN - 0168-3659

IS - 2

ER -

ID: 41884468