Polymeric nanoparticles as an oral delivery system for biocontrol agents for the brushtail possum (trichosurus vulpecula)
Research output: Contribution to journal › Journal article › peer-review
Aim: To investigate polymeric nanoparticles as an oral delivery system for protein biocontrol agents for control of the brushtail possum, Trichosurus vulpecula. Methods: Insulin-loaded poly(ethyl 2-cyanoacrylate) (PECA) nanoparticles were prepared using interfacial polymerisation, and characterised for size, zeta potential, and efficiency of encapsulation of insulin. In-vitro release of insulin-loaded PECA nanoparticles was quantified using reverse-phase highpressure liquid chromatography (HPLC). The in-vivo pharmacokinetics of insulin in PECA nanoparticles was investigated following I/V administration, and when injected directly into the caecum alone or in conjunction with the permeation enhancer EDTA. Blood samples were collected at intervals from −5 to 180 minutes, and the concentration of insulin in plasma was quantified using a radioimmunoassay (RIA) validated for possum plasma. Results: Poly(ethyl 2-cyanoacrylate) nanoparticles were produced with a uniform particle size of 200–300 nm, and the mean entrapment of insulin was 78%. In-vitro release of insulin from the PECA nanoparticles was controlled, although incomplete, and approximately 30% of the insulin remained entrapped. The bioavailability of insulin when administered in a PECA nanoparticulate formulation injected directly into the caecum was <1%, and was not increased by addition of the permeation enhancer. Conclusions: The nanoparticulate formulations investigated as part of this study resulted in low bioavailability of the peptide insulin in the brushtail possum.
Original language | English |
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Journal | New Zealand Veterinary Journal |
Volume | 57 |
Issue number | 6 |
Pages (from-to) | 370-377 |
Number of pages | 8 |
ISSN | 0048-0169 |
DOIs | |
Publication status | Published - Dec 2009 |
- Biological control, Brushtail possum, Insulin, Nanoparticles, Oral delivery, Pharmacokinetics, Radioimmunoassay, Trichosurus vulpecula
Research areas
ID: 299427787