Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene. / Cirera, Susanna; Hillig, Ann-Britt Nygaard; Jensen, Henrik Michael Elvang; Skovgaard, K.; Boye, M.; Fredholm, Merete.

In: Genomics, Vol. 88, No. 5, 2006, p. 659-668.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Cirera, S, Hillig, A-BN, Jensen, HME, Skovgaard, K, Boye, M & Fredholm, M 2006, 'Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene', Genomics, vol. 88, no. 5, pp. 659-668. https://doi.org/10.1016/j.ygeno.2006.04.011

APA

Cirera, S., Hillig, A-B. N., Jensen, H. M. E., Skovgaard, K., Boye, M., & Fredholm, M. (2006). Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene. Genomics, 88(5), 659-668. https://doi.org/10.1016/j.ygeno.2006.04.011

Vancouver

Cirera S, Hillig A-BN, Jensen HME, Skovgaard K, Boye M, Fredholm M. Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene. Genomics. 2006;88(5):659-668. https://doi.org/10.1016/j.ygeno.2006.04.011

Author

Cirera, Susanna ; Hillig, Ann-Britt Nygaard ; Jensen, Henrik Michael Elvang ; Skovgaard, K. ; Boye, M. ; Fredholm, Merete. / Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene. In: Genomics. 2006 ; Vol. 88, No. 5. pp. 659-668.

Bibtex

@article{e62a0850a1c011ddb6ae000ea68e967b,
title = "Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene",
abstract = "The surfactant, pulmonary-associated protein C (SFTPC) is a peptide secreted by the alveolar type II pneumocytes of the lung. We have characterized the porcine SFTPC gene at genomic, transcriptional, and protein levels. The porcine SFTPC is a single-copy gene on pig chromosome 14. Two transcripts were found in a newborn pig lung cDNA library: a full-length clone and a clone missing exon 5. cDNA sequence comparison revealed four synonymous and two nonsynonymous substitutions and in-frame insertions at the beginning of exon 5. Comparison of the SFTPC coding region between several mammals showed high levels of conservation. Northern blot studies showed lung-specific expression of the full-length SFTPC transcript, appearing in 50-day-old fetus and increasing during lung development. Both SFTPC transcripts were detected mainly in lung by real-time RT-PCR and they were significantly down-regulated in necrotic lungs of pigs infected with Actinobacillus pleuropneumoniae. Additionally, the protein levels were also decreased or absent in the necrotic tissue.",
keywords = "Former LIFE faculty, SFTPC, SNPs, gene expression, real-time RT-PCR, Actinobacillus pleuropneumoniae lung infection, type II cells",
author = "Susanna Cirera and Hillig, {Ann-Britt Nygaard} and Jensen, {Henrik Michael Elvang} and K. Skovgaard and M. Boye and Merete Fredholm",
year = "2006",
doi = "10.1016/j.ygeno.2006.04.011",
language = "English",
volume = "88",
pages = "659--668",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press",
number = "5",

}

RIS

TY - JOUR

T1 - Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene

AU - Cirera, Susanna

AU - Hillig, Ann-Britt Nygaard

AU - Jensen, Henrik Michael Elvang

AU - Skovgaard, K.

AU - Boye, M.

AU - Fredholm, Merete

PY - 2006

Y1 - 2006

N2 - The surfactant, pulmonary-associated protein C (SFTPC) is a peptide secreted by the alveolar type II pneumocytes of the lung. We have characterized the porcine SFTPC gene at genomic, transcriptional, and protein levels. The porcine SFTPC is a single-copy gene on pig chromosome 14. Two transcripts were found in a newborn pig lung cDNA library: a full-length clone and a clone missing exon 5. cDNA sequence comparison revealed four synonymous and two nonsynonymous substitutions and in-frame insertions at the beginning of exon 5. Comparison of the SFTPC coding region between several mammals showed high levels of conservation. Northern blot studies showed lung-specific expression of the full-length SFTPC transcript, appearing in 50-day-old fetus and increasing during lung development. Both SFTPC transcripts were detected mainly in lung by real-time RT-PCR and they were significantly down-regulated in necrotic lungs of pigs infected with Actinobacillus pleuropneumoniae. Additionally, the protein levels were also decreased or absent in the necrotic tissue.

AB - The surfactant, pulmonary-associated protein C (SFTPC) is a peptide secreted by the alveolar type II pneumocytes of the lung. We have characterized the porcine SFTPC gene at genomic, transcriptional, and protein levels. The porcine SFTPC is a single-copy gene on pig chromosome 14. Two transcripts were found in a newborn pig lung cDNA library: a full-length clone and a clone missing exon 5. cDNA sequence comparison revealed four synonymous and two nonsynonymous substitutions and in-frame insertions at the beginning of exon 5. Comparison of the SFTPC coding region between several mammals showed high levels of conservation. Northern blot studies showed lung-specific expression of the full-length SFTPC transcript, appearing in 50-day-old fetus and increasing during lung development. Both SFTPC transcripts were detected mainly in lung by real-time RT-PCR and they were significantly down-regulated in necrotic lungs of pigs infected with Actinobacillus pleuropneumoniae. Additionally, the protein levels were also decreased or absent in the necrotic tissue.

KW - Former LIFE faculty

KW - SFTPC, SNPs, gene expression, real-time RT-PCR

KW - Actinobacillus pleuropneumoniae lung infection

KW - type II cells

U2 - 10.1016/j.ygeno.2006.04.011

DO - 10.1016/j.ygeno.2006.04.011

M3 - Journal article

C2 - 16769199

VL - 88

SP - 659

EP - 668

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 5

ER -

ID: 8026436