TY - JOUR

T1 - Microcontainers as an oral delivery system for spray dried cubosomes containing ovalbumin

AU - Nielsen, Line Hagner

AU - Rades, Thomas

AU - Boyd, Ben J

AU - Boisen, Anja

PY - 2017/9

Y1 - 2017/9

N2 - The purpose of this study was to prepare cubosomes encapsulating the model antigen ovalbumin (OVA) via spray drying, and to characterise such cubosomes with a view for their potential application in oral vaccine delivery. Furthermore the cubosome formulation was loaded into polymeric microcontainers intended as an oral drug delivery system. The cubosomes consisted of commercial glyceryl monooleate, Dimodan®, containing OVA and were surrounded with a dextran shell prepared by spray drying. Cryo-TEM was used to confirm that cubosomes were formed after hydration of the spray dried precursor powder. The precursor powder had a mean particle size of 1.3. ±. 0.1. μm, whereas the mean diameter of the dispersed cubosomes was 282. ±. 7. nm (PDI: 0.18) measured by dynamic light scattering. 8.5. ±. 0.3% (w/w) of OVA was present in the cubosome powder and OVA was found released slowly over the first 70. h, followed by a more rapid release. Total release of 47.9. ±. 2.8% of loaded OVA occurred over 96. h in a buffer at pH 6.8. When the powder was filled into microcontainers, and the opening covered with the pH sensitive polymer Eudragit S100, the pH sensitive 'lid' was intact at gastric pH, but release of OVA from the cubosomes and microcontainers occurred at pH 6.8, releasing 44.1. ±. 5.6% of the OVA in 96. h. Small-angle X-ray scattering (SAXS) revealed that the 'dry' particles possessed an internal ordered lipid structure (lamellar and inverse micellar phase) by virtue of a small amount of residual water, and after hydration in buffer at pH 6.8, the particles formed the hexagonal inverse cubic phases, thereby indicating that cubosomes were formed when released from microcontainers.

AB - The purpose of this study was to prepare cubosomes encapsulating the model antigen ovalbumin (OVA) via spray drying, and to characterise such cubosomes with a view for their potential application in oral vaccine delivery. Furthermore the cubosome formulation was loaded into polymeric microcontainers intended as an oral drug delivery system. The cubosomes consisted of commercial glyceryl monooleate, Dimodan®, containing OVA and were surrounded with a dextran shell prepared by spray drying. Cryo-TEM was used to confirm that cubosomes were formed after hydration of the spray dried precursor powder. The precursor powder had a mean particle size of 1.3. ±. 0.1. μm, whereas the mean diameter of the dispersed cubosomes was 282. ±. 7. nm (PDI: 0.18) measured by dynamic light scattering. 8.5. ±. 0.3% (w/w) of OVA was present in the cubosome powder and OVA was found released slowly over the first 70. h, followed by a more rapid release. Total release of 47.9. ±. 2.8% of loaded OVA occurred over 96. h in a buffer at pH 6.8. When the powder was filled into microcontainers, and the opening covered with the pH sensitive polymer Eudragit S100, the pH sensitive 'lid' was intact at gastric pH, but release of OVA from the cubosomes and microcontainers occurred at pH 6.8, releasing 44.1. ±. 5.6% of the OVA in 96. h. Small-angle X-ray scattering (SAXS) revealed that the 'dry' particles possessed an internal ordered lipid structure (lamellar and inverse micellar phase) by virtue of a small amount of residual water, and after hydration in buffer at pH 6.8, the particles formed the hexagonal inverse cubic phases, thereby indicating that cubosomes were formed when released from microcontainers.

KW - Lipid self-assembly

KW - Micro devices

KW - Oral drug delivery

KW - Oral vaccine delivery

KW - Particulates

KW - Spray drying

U2 - 10.1016/j.ejpb.2016.12.008

DO - 10.1016/j.ejpb.2016.12.008

M3 - Journal article

C2 - 27993733

AN - SCOPUS:85010807569

VL - 118

SP - 13

EP - 20

JO - European Journal of Pharmaceutics and Biopharmaceutics

JF - European Journal of Pharmaceutics and Biopharmaceutics

SN - 0939-6411

ER -