Method development and characterisation of the low-molecular-weight peptidome of human wound fluids

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Method development and characterisation of the low-molecular-weight peptidome of human wound fluids. / Plas, Mariena JA van der; Cai, Jun; Petrlova, Jitka; Saleh, Karim; Kjellström, Sven; Schmidtchen, Artur.

In: eLife, Vol. 10, e66876, 06.07.2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Plas, MJAVD, Cai, J, Petrlova, J, Saleh, K, Kjellström, S & Schmidtchen, A 2021, 'Method development and characterisation of the low-molecular-weight peptidome of human wound fluids', eLife, vol. 10, e66876. https://doi.org/10.7554/eLife.66876

APA

Plas, M. JA. V. D., Cai, J., Petrlova, J., Saleh, K., Kjellström, S., & Schmidtchen, A. (2021). Method development and characterisation of the low-molecular-weight peptidome of human wound fluids. eLife, 10, [e66876]. https://doi.org/10.7554/eLife.66876

Vancouver

Plas MJAVD, Cai J, Petrlova J, Saleh K, Kjellström S, Schmidtchen A. Method development and characterisation of the low-molecular-weight peptidome of human wound fluids. eLife. 2021 Jul 6;10. e66876. https://doi.org/10.7554/eLife.66876

Author

Plas, Mariena JA van der ; Cai, Jun ; Petrlova, Jitka ; Saleh, Karim ; Kjellström, Sven ; Schmidtchen, Artur. / Method development and characterisation of the low-molecular-weight peptidome of human wound fluids. In: eLife. 2021 ; Vol. 10.

Bibtex

@article{3035def6bca34befa98abd662b02df0f,
title = "Method development and characterisation of the low-molecular-weight peptidome of human wound fluids",
abstract = "The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using mass-spectrometry analyses, we here for the first time characterise the peptidome of human wound fluids. Sterile post-surgical wound fluids were found to contain a high degree of peptides in comparison to human plasma. Analyses of the peptidome from uninfected healing wounds and Staphylococcus aureus -infected wounds identify unique peptide patterns of various proteins, including coagulation and complement factors, proteases, and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from wound fluids and demonstrates a proof-of-concept that such fluids can be used for analysis of qualitative differences of peptide patterns from larger patient cohorts, providing potential biomarkers for wound healing and infection.",
author = "Plas, {Mariena JA van der} and Jun Cai and Jitka Petrlova and Karim Saleh and Sven Kjellstr{\"o}m and Artur Schmidtchen",
year = "2021",
month = jul,
day = "6",
doi = "10.7554/eLife.66876",
language = "English",
volume = "10",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications Ltd.",

}

RIS

TY - JOUR

T1 - Method development and characterisation of the low-molecular-weight peptidome of human wound fluids

AU - Plas, Mariena JA van der

AU - Cai, Jun

AU - Petrlova, Jitka

AU - Saleh, Karim

AU - Kjellström, Sven

AU - Schmidtchen, Artur

PY - 2021/7/6

Y1 - 2021/7/6

N2 - The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using mass-spectrometry analyses, we here for the first time characterise the peptidome of human wound fluids. Sterile post-surgical wound fluids were found to contain a high degree of peptides in comparison to human plasma. Analyses of the peptidome from uninfected healing wounds and Staphylococcus aureus -infected wounds identify unique peptide patterns of various proteins, including coagulation and complement factors, proteases, and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from wound fluids and demonstrates a proof-of-concept that such fluids can be used for analysis of qualitative differences of peptide patterns from larger patient cohorts, providing potential biomarkers for wound healing and infection.

AB - The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using mass-spectrometry analyses, we here for the first time characterise the peptidome of human wound fluids. Sterile post-surgical wound fluids were found to contain a high degree of peptides in comparison to human plasma. Analyses of the peptidome from uninfected healing wounds and Staphylococcus aureus -infected wounds identify unique peptide patterns of various proteins, including coagulation and complement factors, proteases, and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from wound fluids and demonstrates a proof-of-concept that such fluids can be used for analysis of qualitative differences of peptide patterns from larger patient cohorts, providing potential biomarkers for wound healing and infection.

U2 - 10.7554/eLife.66876

DO - 10.7554/eLife.66876

M3 - Journal article

C2 - 34227939

VL - 10

JO - eLife

JF - eLife

SN - 2050-084X

M1 - e66876

ER -

ID: 273640178