Mapping the intermediate digestion phases of human healthy intestinal contents from distal ileum and caecum at fasted and fed state conditions

Research output: Contribution to journalJournal articleResearchpeer-review

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Mapping the intermediate digestion phases of human healthy intestinal contents from distal ileum and caecum at fasted and fed state conditions. / Tran, Thuy; Fatouros, Dimitrios G; Vertzoni, Maria; Reppas, Christos; Müllertz, Anette.

In: The Journal of pharmacy and pharmacology, Vol. 69, No. 3, 03.2017, p. 265-273.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Tran, T, Fatouros, DG, Vertzoni, M, Reppas, C & Müllertz, A 2017, 'Mapping the intermediate digestion phases of human healthy intestinal contents from distal ileum and caecum at fasted and fed state conditions', The Journal of pharmacy and pharmacology, vol. 69, no. 3, pp. 265-273. https://doi.org/10.1111/jphp.12686

APA

Tran, T., Fatouros, D. G., Vertzoni, M., Reppas, C., & Müllertz, A. (2017). Mapping the intermediate digestion phases of human healthy intestinal contents from distal ileum and caecum at fasted and fed state conditions. The Journal of pharmacy and pharmacology, 69(3), 265-273. https://doi.org/10.1111/jphp.12686

Vancouver

Tran T, Fatouros DG, Vertzoni M, Reppas C, Müllertz A. Mapping the intermediate digestion phases of human healthy intestinal contents from distal ileum and caecum at fasted and fed state conditions. The Journal of pharmacy and pharmacology. 2017 Mar;69(3):265-273. https://doi.org/10.1111/jphp.12686

Author

Tran, Thuy ; Fatouros, Dimitrios G ; Vertzoni, Maria ; Reppas, Christos ; Müllertz, Anette. / Mapping the intermediate digestion phases of human healthy intestinal contents from distal ileum and caecum at fasted and fed state conditions. In: The Journal of pharmacy and pharmacology. 2017 ; Vol. 69, No. 3. pp. 265-273.

Bibtex

@article{c5af40312d2d4b1fbceb3ea6b65cc8f7,
title = "Mapping the intermediate digestion phases of human healthy intestinal contents from distal ileum and caecum at fasted and fed state conditions",
abstract = "OBJECTIVES: To investigate at the ultrastructural level, the colloidal phases formed in the lumen of the distal ileum and caecum of healthy adults.METHODS: Cryogenic transmission electron microscopy (Cryo-TEM) was employed to image the intermediate colloidal phases of human intestinal contents collected from distal ileum and caecum of two healthy volunteers under fasted and fed state conditions.KEY FINDINGS: In samples collected both in the fasted and fed states, Cryo-TEM study revealed the presence of large spherical unilamellar and occasionally bi-lamellar and oligolamellar vesicles with diameters ranging from 50 to 200 nm for both volunteers in distal ileum and caecum. Bilayer fragments were frequently observed in caecal samples. Plate-like structures resembling the morphology of cholesterol plates were visualised in all samples. Elongated structures were observed in the fed state in distal ileum and caecum for both volunteers, whereas no micellar structures could be detected for all samples.CONCLUSIONS: This study provides a framework for understanding the structure of colloidal phases, and it may assist in elucidating the role of dosing conditions on drug absorption from the distal ileum and caecum.",
keywords = "Cecum, Cholesterol, Fasting, Gastrointestinal Contents, Humans, Ileum, Intestinal Secretions, Microscopy, Electron, Transmission, Journal Article",
author = "Thuy Tran and Fatouros, {Dimitrios G} and Maria Vertzoni and Christos Reppas and Anette M{\"u}llertz",
note = "{\circledC} 2017 Royal Pharmaceutical Society.",
year = "2017",
month = "3",
doi = "10.1111/jphp.12686",
language = "English",
volume = "69",
pages = "265--273",
journal = "Journal of Pharmacy and Pharmacology",
issn = "0022-3573",
publisher = "JohnWiley & Sons Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - Mapping the intermediate digestion phases of human healthy intestinal contents from distal ileum and caecum at fasted and fed state conditions

AU - Tran, Thuy

AU - Fatouros, Dimitrios G

AU - Vertzoni, Maria

AU - Reppas, Christos

AU - Müllertz, Anette

N1 - © 2017 Royal Pharmaceutical Society.

PY - 2017/3

Y1 - 2017/3

N2 - OBJECTIVES: To investigate at the ultrastructural level, the colloidal phases formed in the lumen of the distal ileum and caecum of healthy adults.METHODS: Cryogenic transmission electron microscopy (Cryo-TEM) was employed to image the intermediate colloidal phases of human intestinal contents collected from distal ileum and caecum of two healthy volunteers under fasted and fed state conditions.KEY FINDINGS: In samples collected both in the fasted and fed states, Cryo-TEM study revealed the presence of large spherical unilamellar and occasionally bi-lamellar and oligolamellar vesicles with diameters ranging from 50 to 200 nm for both volunteers in distal ileum and caecum. Bilayer fragments were frequently observed in caecal samples. Plate-like structures resembling the morphology of cholesterol plates were visualised in all samples. Elongated structures were observed in the fed state in distal ileum and caecum for both volunteers, whereas no micellar structures could be detected for all samples.CONCLUSIONS: This study provides a framework for understanding the structure of colloidal phases, and it may assist in elucidating the role of dosing conditions on drug absorption from the distal ileum and caecum.

AB - OBJECTIVES: To investigate at the ultrastructural level, the colloidal phases formed in the lumen of the distal ileum and caecum of healthy adults.METHODS: Cryogenic transmission electron microscopy (Cryo-TEM) was employed to image the intermediate colloidal phases of human intestinal contents collected from distal ileum and caecum of two healthy volunteers under fasted and fed state conditions.KEY FINDINGS: In samples collected both in the fasted and fed states, Cryo-TEM study revealed the presence of large spherical unilamellar and occasionally bi-lamellar and oligolamellar vesicles with diameters ranging from 50 to 200 nm for both volunteers in distal ileum and caecum. Bilayer fragments were frequently observed in caecal samples. Plate-like structures resembling the morphology of cholesterol plates were visualised in all samples. Elongated structures were observed in the fed state in distal ileum and caecum for both volunteers, whereas no micellar structures could be detected for all samples.CONCLUSIONS: This study provides a framework for understanding the structure of colloidal phases, and it may assist in elucidating the role of dosing conditions on drug absorption from the distal ileum and caecum.

KW - Cecum

KW - Cholesterol

KW - Fasting

KW - Gastrointestinal Contents

KW - Humans

KW - Ileum

KW - Intestinal Secretions

KW - Microscopy, Electron, Transmission

KW - Journal Article

U2 - 10.1111/jphp.12686

DO - 10.1111/jphp.12686

M3 - Journal article

C2 - 28106271

VL - 69

SP - 265

EP - 273

JO - Journal of Pharmacy and Pharmacology

JF - Journal of Pharmacy and Pharmacology

SN - 0022-3573

IS - 3

ER -

ID: 185745322