Lipoprotein ability to exchange and remove lipids from model membranes as a function of fatty acid saturation and presence of cholesterol

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Lipoprotein ability to exchange and remove lipids from model membranes as a function of fatty acid saturation and presence of cholesterol. / Waldie, Sarah; Sebastiani, Federica; Browning, Kathryn; Maric, Selma; Lind, Tania K.; Yepuri, Nageshwar; Darwish, Tamim A.; Moulin, Martine; Strohmeier, Gernot; Pichler, Harald; Skoda, Maximilian W. A.; Maestro, Armando; Haertlein, Michael; Forsyth, V. Trevor; Bengtsson, Eva; Malmsten, Martin; Cardenas, Marite.

In: B B A - Molecular and Cell Biology of Lipids, Vol. 1865, No. 10, 158769, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Waldie, S, Sebastiani, F, Browning, K, Maric, S, Lind, TK, Yepuri, N, Darwish, TA, Moulin, M, Strohmeier, G, Pichler, H, Skoda, MWA, Maestro, A, Haertlein, M, Forsyth, VT, Bengtsson, E, Malmsten, M & Cardenas, M 2020, 'Lipoprotein ability to exchange and remove lipids from model membranes as a function of fatty acid saturation and presence of cholesterol', B B A - Molecular and Cell Biology of Lipids, vol. 1865, no. 10, 158769. https://doi.org/10.1016/j.bbalip.2020.158769

APA

Waldie, S., Sebastiani, F., Browning, K., Maric, S., Lind, T. K., Yepuri, N., Darwish, T. A., Moulin, M., Strohmeier, G., Pichler, H., Skoda, M. W. A., Maestro, A., Haertlein, M., Forsyth, V. T., Bengtsson, E., Malmsten, M., & Cardenas, M. (2020). Lipoprotein ability to exchange and remove lipids from model membranes as a function of fatty acid saturation and presence of cholesterol. B B A - Molecular and Cell Biology of Lipids, 1865(10), [158769]. https://doi.org/10.1016/j.bbalip.2020.158769

Vancouver

Waldie S, Sebastiani F, Browning K, Maric S, Lind TK, Yepuri N et al. Lipoprotein ability to exchange and remove lipids from model membranes as a function of fatty acid saturation and presence of cholesterol. B B A - Molecular and Cell Biology of Lipids. 2020;1865(10). 158769. https://doi.org/10.1016/j.bbalip.2020.158769

Author

Waldie, Sarah ; Sebastiani, Federica ; Browning, Kathryn ; Maric, Selma ; Lind, Tania K. ; Yepuri, Nageshwar ; Darwish, Tamim A. ; Moulin, Martine ; Strohmeier, Gernot ; Pichler, Harald ; Skoda, Maximilian W. A. ; Maestro, Armando ; Haertlein, Michael ; Forsyth, V. Trevor ; Bengtsson, Eva ; Malmsten, Martin ; Cardenas, Marite. / Lipoprotein ability to exchange and remove lipids from model membranes as a function of fatty acid saturation and presence of cholesterol. In: B B A - Molecular and Cell Biology of Lipids. 2020 ; Vol. 1865, No. 10.

Bibtex

@article{2517c0c064c445e9a81551eeea4393e1,
title = "Lipoprotein ability to exchange and remove lipids from model membranes as a function of fatty acid saturation and presence of cholesterol",
abstract = "Lipoproteins play a central role in the development of atherosclerosis. High and low-density lipoproteins (HDL and LDL), known as 'good' and 'bad' cholesterol, respectively, remove and/or deposit lipids into the artery wall. Hence, insight into lipid exchange processes between lipoproteins and cell membranes is of particular importance in understanding the onset and development of cardiovascular disease. In order to elucidate the impact of phospholipid tail saturation and the presence of cholesterol in cell membranes on these processes, neutron reflection was employed in the present investigation to follow lipid exchange with both HDL and LDL against model membranes. Mirroring clinical risk factors for the development of atherosclerosis, lower exchange was observed in the presence of cholesterol, as well as for an unsaturated phospholipid, compared to faster exchange when using a fully saturated phospholipid. These results highlight the importance of membrane composition on the interaction with lipoproteins, chiefly the saturation level of the lipids and presence of cholesterol, and provide novel insight into factors of importance for build-up and reversibility of atherosclerotic plaque. In addition, the correlation between the results and well-established clinical risk factors suggests that the approach taken can be employed also for understanding a broader set of risk factors including, e.g., effects of triglycerides and oxidative stress, as well as local effects of drugs on atherosclerotic plaque formation.",
keywords = "Lipoproteins, Cholesterol, Neutron reflection, Lipid removal, Saturated fats, LOW-DENSITY LIPOPROTEINS, APOLIPOPROTEIN B-100, HDL-CHOLESTEROL, NEUTRON-SCATTERING, LATERAL DIFFUSION, A-I, BILAYER, LDL, DIMYRISTOYLPHOSPHATIDYLCHOLINE, DEUTERATION",
author = "Sarah Waldie and Federica Sebastiani and Kathryn Browning and Selma Maric and Lind, {Tania K.} and Nageshwar Yepuri and Darwish, {Tamim A.} and Martine Moulin and Gernot Strohmeier and Harald Pichler and Skoda, {Maximilian W. A.} and Armando Maestro and Michael Haertlein and Forsyth, {V. Trevor} and Eva Bengtsson and Martin Malmsten and Marite Cardenas",
year = "2020",
doi = "10.1016/j.bbalip.2020.158769",
language = "English",
volume = "1865",
journal = "B B A - Molecular and Cell Biology of Lipids",
issn = "1388-1981",
publisher = "Elsevier",
number = "10",

}

RIS

TY - JOUR

T1 - Lipoprotein ability to exchange and remove lipids from model membranes as a function of fatty acid saturation and presence of cholesterol

AU - Waldie, Sarah

AU - Sebastiani, Federica

AU - Browning, Kathryn

AU - Maric, Selma

AU - Lind, Tania K.

AU - Yepuri, Nageshwar

AU - Darwish, Tamim A.

AU - Moulin, Martine

AU - Strohmeier, Gernot

AU - Pichler, Harald

AU - Skoda, Maximilian W. A.

AU - Maestro, Armando

AU - Haertlein, Michael

AU - Forsyth, V. Trevor

AU - Bengtsson, Eva

AU - Malmsten, Martin

AU - Cardenas, Marite

PY - 2020

Y1 - 2020

N2 - Lipoproteins play a central role in the development of atherosclerosis. High and low-density lipoproteins (HDL and LDL), known as 'good' and 'bad' cholesterol, respectively, remove and/or deposit lipids into the artery wall. Hence, insight into lipid exchange processes between lipoproteins and cell membranes is of particular importance in understanding the onset and development of cardiovascular disease. In order to elucidate the impact of phospholipid tail saturation and the presence of cholesterol in cell membranes on these processes, neutron reflection was employed in the present investigation to follow lipid exchange with both HDL and LDL against model membranes. Mirroring clinical risk factors for the development of atherosclerosis, lower exchange was observed in the presence of cholesterol, as well as for an unsaturated phospholipid, compared to faster exchange when using a fully saturated phospholipid. These results highlight the importance of membrane composition on the interaction with lipoproteins, chiefly the saturation level of the lipids and presence of cholesterol, and provide novel insight into factors of importance for build-up and reversibility of atherosclerotic plaque. In addition, the correlation between the results and well-established clinical risk factors suggests that the approach taken can be employed also for understanding a broader set of risk factors including, e.g., effects of triglycerides and oxidative stress, as well as local effects of drugs on atherosclerotic plaque formation.

AB - Lipoproteins play a central role in the development of atherosclerosis. High and low-density lipoproteins (HDL and LDL), known as 'good' and 'bad' cholesterol, respectively, remove and/or deposit lipids into the artery wall. Hence, insight into lipid exchange processes between lipoproteins and cell membranes is of particular importance in understanding the onset and development of cardiovascular disease. In order to elucidate the impact of phospholipid tail saturation and the presence of cholesterol in cell membranes on these processes, neutron reflection was employed in the present investigation to follow lipid exchange with both HDL and LDL against model membranes. Mirroring clinical risk factors for the development of atherosclerosis, lower exchange was observed in the presence of cholesterol, as well as for an unsaturated phospholipid, compared to faster exchange when using a fully saturated phospholipid. These results highlight the importance of membrane composition on the interaction with lipoproteins, chiefly the saturation level of the lipids and presence of cholesterol, and provide novel insight into factors of importance for build-up and reversibility of atherosclerotic plaque. In addition, the correlation between the results and well-established clinical risk factors suggests that the approach taken can be employed also for understanding a broader set of risk factors including, e.g., effects of triglycerides and oxidative stress, as well as local effects of drugs on atherosclerotic plaque formation.

KW - Lipoproteins

KW - Cholesterol

KW - Neutron reflection

KW - Lipid removal

KW - Saturated fats

KW - LOW-DENSITY LIPOPROTEINS

KW - APOLIPOPROTEIN B-100

KW - HDL-CHOLESTEROL

KW - NEUTRON-SCATTERING

KW - LATERAL DIFFUSION

KW - A-I

KW - BILAYER

KW - LDL

KW - DIMYRISTOYLPHOSPHATIDYLCHOLINE

KW - DEUTERATION

U2 - 10.1016/j.bbalip.2020.158769

DO - 10.1016/j.bbalip.2020.158769

M3 - Journal article

C2 - 32712249

VL - 1865

JO - B B A - Molecular and Cell Biology of Lipids

JF - B B A - Molecular and Cell Biology of Lipids

SN - 1388-1981

IS - 10

M1 - 158769

ER -

ID: 249300922