Kinetics of insulin adsorption at the oil-water interface and diffusion properties of adsorbed layers monitored using fluorescence correlation spectroscopy

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Kinetics of insulin adsorption at the oil-water interface and diffusion properties of adsorbed layers monitored using fluorescence correlation spectroscopy. / Donsmark, Jesper; Jorgensen, Lene; Mollmann, Susanne; Frokjaer, Sven; Rischel, Christian.

In: Pharmaceutical Research, Vol. 23, No. 1, 01.01.2006, p. 148-155.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Donsmark, J, Jorgensen, L, Mollmann, S, Frokjaer, S & Rischel, C 2006, 'Kinetics of insulin adsorption at the oil-water interface and diffusion properties of adsorbed layers monitored using fluorescence correlation spectroscopy', Pharmaceutical Research, vol. 23, no. 1, pp. 148-155. https://doi.org/10.1007/s11095-005-8636-3

APA

Donsmark, J., Jorgensen, L., Mollmann, S., Frokjaer, S., & Rischel, C. (2006). Kinetics of insulin adsorption at the oil-water interface and diffusion properties of adsorbed layers monitored using fluorescence correlation spectroscopy. Pharmaceutical Research, 23(1), 148-155. https://doi.org/10.1007/s11095-005-8636-3

Vancouver

Donsmark J, Jorgensen L, Mollmann S, Frokjaer S, Rischel C. Kinetics of insulin adsorption at the oil-water interface and diffusion properties of adsorbed layers monitored using fluorescence correlation spectroscopy. Pharmaceutical Research. 2006 Jan 1;23(1):148-155. https://doi.org/10.1007/s11095-005-8636-3

Author

Donsmark, Jesper ; Jorgensen, Lene ; Mollmann, Susanne ; Frokjaer, Sven ; Rischel, Christian. / Kinetics of insulin adsorption at the oil-water interface and diffusion properties of adsorbed layers monitored using fluorescence correlation spectroscopy. In: Pharmaceutical Research. 2006 ; Vol. 23, No. 1. pp. 148-155.

Bibtex

@article{578376d6ddb8496ab3c677b18172bedb,
title = "Kinetics of insulin adsorption at the oil-water interface and diffusion properties of adsorbed layers monitored using fluorescence correlation spectroscopy",
abstract = "The adsorption of insulin at an oil-water interface was studied with fluorescence correlation spectroscopy (FCS). FCS is able to measure diffusion properties of insulin at nanomolar concentrations, making it possible to detect the very early steps in the adsorption process. Below 20 nM bulk insulin concentration, the insulin molecules adsorbed to the surface diffuse freely at all times during the experiment (a few hours). At higher concentrations, a surprisingly abrupt transition to a slow diffusion phase is observed. Based on the information about both diffusion times and molecular brightness derived from the FCS experiments, we suggest that the transition represents the formation of a fractal network. FCS may be a valuable tool in pharmaceutical formulation science, because it provides information about concentration buildup and phase changes at interfaces formed in drug delivery systems.",
keywords = "Adsorption, Fluorescence, Fluorescence correlation spectroscopy, Insulin, Oil-water interface",
author = "Jesper Donsmark and Lene Jorgensen and Susanne Mollmann and Sven Frokjaer and Christian Rischel",
year = "2006",
month = jan,
day = "1",
doi = "10.1007/s11095-005-8636-3",
language = "English",
volume = "23",
pages = "148--155",
journal = "Pharmaceutical Research",
issn = "0724-8741",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - Kinetics of insulin adsorption at the oil-water interface and diffusion properties of adsorbed layers monitored using fluorescence correlation spectroscopy

AU - Donsmark, Jesper

AU - Jorgensen, Lene

AU - Mollmann, Susanne

AU - Frokjaer, Sven

AU - Rischel, Christian

PY - 2006/1/1

Y1 - 2006/1/1

N2 - The adsorption of insulin at an oil-water interface was studied with fluorescence correlation spectroscopy (FCS). FCS is able to measure diffusion properties of insulin at nanomolar concentrations, making it possible to detect the very early steps in the adsorption process. Below 20 nM bulk insulin concentration, the insulin molecules adsorbed to the surface diffuse freely at all times during the experiment (a few hours). At higher concentrations, a surprisingly abrupt transition to a slow diffusion phase is observed. Based on the information about both diffusion times and molecular brightness derived from the FCS experiments, we suggest that the transition represents the formation of a fractal network. FCS may be a valuable tool in pharmaceutical formulation science, because it provides information about concentration buildup and phase changes at interfaces formed in drug delivery systems.

AB - The adsorption of insulin at an oil-water interface was studied with fluorescence correlation spectroscopy (FCS). FCS is able to measure diffusion properties of insulin at nanomolar concentrations, making it possible to detect the very early steps in the adsorption process. Below 20 nM bulk insulin concentration, the insulin molecules adsorbed to the surface diffuse freely at all times during the experiment (a few hours). At higher concentrations, a surprisingly abrupt transition to a slow diffusion phase is observed. Based on the information about both diffusion times and molecular brightness derived from the FCS experiments, we suggest that the transition represents the formation of a fractal network. FCS may be a valuable tool in pharmaceutical formulation science, because it provides information about concentration buildup and phase changes at interfaces formed in drug delivery systems.

KW - Adsorption

KW - Fluorescence

KW - Fluorescence correlation spectroscopy

KW - Insulin

KW - Oil-water interface

UR - http://www.scopus.com/inward/record.url?scp=32244435209&partnerID=8YFLogxK

U2 - 10.1007/s11095-005-8636-3

DO - 10.1007/s11095-005-8636-3

M3 - Journal article

C2 - 16307385

AN - SCOPUS:32244435209

VL - 23

SP - 148

EP - 155

JO - Pharmaceutical Research

JF - Pharmaceutical Research

SN - 0724-8741

IS - 1

ER -

ID: 208959085