Isolation, structural elucidation and in vitro activity of 2-acetyl-2-decarboxamido-oxytetracycline against environmental relevant bacteria, including tetracycline-resistant bacteria
Research output: Contribution to journal › Journal article › Research › peer-review
2-Acetyl-2-decarboxamido-oxytetracycline (ADOTC) is a major impurity of oxytetracycline (OTC) produced as a side product during fermentation. ADOTC was isolated from OTC and other impurities using preparative HPLC. The preparative column was an Xterra MS, C18 chromatographic column (100 mm x 19 mm i.d., 5 microm), and the mobile phase contained methanol-water (27:73 (v/v)) with 0.08 M formic acid added. The flow rate was 9.0 ml/min. It was possible to isolate few milligram ADOTC in a day. The compound was unambiguously identified using NMR and MS-MS. The anti-microbial activity against activated sludge bacteria was determined giving a potency of only 3% of that of OTC. With tetracycline-resistant bacteria, no anti-microbial activity was observed, indicating a mode of action similar to that of OTC.
| Original language | English |
|---|---|
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 34 |
| Issue number | 3 |
| Pages (from-to) | 559-67 |
| Number of pages | 8 |
| ISSN | 0731-7085 |
| Publication status | Published - 2004 |
Bibliographical note
Keywords: Animals; Chickens; Chromatography, High Pressure Liquid; Escherichia coli; Microbial Sensitivity Tests; Molecular Structure; Sewage; Swine; Tetracycline Resistance; Tetracyclines
ID: 9040296