Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols. / De Vitis, Stefania; Matarise, Giuseppina; Pardeo, Francesca; Catalano, Rossella; Malara, Natalia; Trunzo, Valentina; Tallerico, Rossana; Gentile, Francesco; Candeloro, Patrizio; Coluccio, Maria Laura; Massaro, Alessandro; Viglietto, Giuseppe; Carbone, Ennio; Kutter, Jörg P.; Perozziello, Gerardo; Di Fabrizio, Enzo.

In: Microelectronic Engineering, Vol. 124, 25.07.2014, p. 76-80.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

De Vitis, S, Matarise, G, Pardeo, F, Catalano, R, Malara, N, Trunzo, V, Tallerico, R, Gentile, F, Candeloro, P, Coluccio, ML, Massaro, A, Viglietto, G, Carbone, E, Kutter, JP, Perozziello, G & Di Fabrizio, E 2014, 'Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols', Microelectronic Engineering, vol. 124, pp. 76-80. https://doi.org/10.1016/j.mee.2014.04.013

APA

De Vitis, S., Matarise, G., Pardeo, F., Catalano, R., Malara, N., Trunzo, V., Tallerico, R., Gentile, F., Candeloro, P., Coluccio, M. L., Massaro, A., Viglietto, G., Carbone, E., Kutter, J. P., Perozziello, G., & Di Fabrizio, E. (2014). Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols. Microelectronic Engineering, 124, 76-80. https://doi.org/10.1016/j.mee.2014.04.013

Vancouver

De Vitis S, Matarise G, Pardeo F, Catalano R, Malara N, Trunzo V et al. Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols. Microelectronic Engineering. 2014 Jul 25;124:76-80. https://doi.org/10.1016/j.mee.2014.04.013

Author

De Vitis, Stefania ; Matarise, Giuseppina ; Pardeo, Francesca ; Catalano, Rossella ; Malara, Natalia ; Trunzo, Valentina ; Tallerico, Rossana ; Gentile, Francesco ; Candeloro, Patrizio ; Coluccio, Maria Laura ; Massaro, Alessandro ; Viglietto, Giuseppe ; Carbone, Ennio ; Kutter, Jörg P. ; Perozziello, Gerardo ; Di Fabrizio, Enzo. / Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols. In: Microelectronic Engineering. 2014 ; Vol. 124. pp. 76-80.

Bibtex

@article{0a8937c282184464b785272c239ff4f0,
title = "Isolation of cancer cells by {"}in situ{"} microfluidic biofunctionalization protocols",
abstract = "The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using {"}in situ{"} microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs.",
keywords = "Biofunctionalization protocols, Cancer cell isolations, Cell sorting, Microfluidic assay",
author = "{De Vitis}, Stefania and Giuseppina Matarise and Francesca Pardeo and Rossella Catalano and Natalia Malara and Valentina Trunzo and Rossana Tallerico and Francesco Gentile and Patrizio Candeloro and Coluccio, {Maria Laura} and Alessandro Massaro and Giuseppe Viglietto and Ennio Carbone and Kutter, {J{\"o}rg P.} and Gerardo Perozziello and {Di Fabrizio}, Enzo",
year = "2014",
month = jul,
day = "25",
doi = "10.1016/j.mee.2014.04.013",
language = "English",
volume = "124",
pages = "76--80",
journal = "Microelectronic Engineering",
issn = "0167-9317",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

AU - De Vitis, Stefania

AU - Matarise, Giuseppina

AU - Pardeo, Francesca

AU - Catalano, Rossella

AU - Malara, Natalia

AU - Trunzo, Valentina

AU - Tallerico, Rossana

AU - Gentile, Francesco

AU - Candeloro, Patrizio

AU - Coluccio, Maria Laura

AU - Massaro, Alessandro

AU - Viglietto, Giuseppe

AU - Carbone, Ennio

AU - Kutter, Jörg P.

AU - Perozziello, Gerardo

AU - Di Fabrizio, Enzo

PY - 2014/7/25

Y1 - 2014/7/25

N2 - The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs.

AB - The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs.

KW - Biofunctionalization protocols

KW - Cancer cell isolations

KW - Cell sorting

KW - Microfluidic assay

UR - http://www.scopus.com/inward/record.url?scp=84901836503&partnerID=8YFLogxK

U2 - 10.1016/j.mee.2014.04.013

DO - 10.1016/j.mee.2014.04.013

M3 - Journal article

AN - SCOPUS:84901836503

VL - 124

SP - 76

EP - 80

JO - Microelectronic Engineering

JF - Microelectronic Engineering

SN - 0167-9317

ER -

ID: 137375185