TY - JOUR

T1 - Interfacing capillary electrophoresis with inductively coupled plasma mass spectrometry by direct injection nebulization for selenium speciation

AU - Bendahl, Lars

AU - Gammelgaard, Bente

AU - Jons, O.

AU - Farver, O.

AU - Hansen, S.H.

PY - 2001

Y1 - 2001

N2 - A demountable direct injection high efficiency nebulizer operating at low sample uptake rates was developed and used for coupling of capillary electrophoresis (CE) with inductively coupled plasma mass spectrometry (ICP-MS). When the nebulizer was used for continuous sample introduction, detection limits of 20 and 1 ng L-1 were obtained for Se-82 and Rh-103, respectively, at sample uptake rates of 10-30 muL min(-1), based on three times the standard deviation of blank solution (3 sigma (b), n = 10). The nebulizer was used as part of the interface for coupling of CE with ICP-MS and applied for speciation of aqueous selenium standards. The interface was operated in the self-aspirating mode with a sheath liquid uptake of 10 muL min(-1). The CE-ICP-MS system resulted in baseline separation of selenate, selenite, selenocystine and selenomethionine within a total analysis time of 5.4 min. Detection limits were in the sub mug Se L-1 range, corresponding to absolute detection limits in the range 25-125 fg selenium. Repeatability (n = 6) expressed as relative standard deviations with respect to migration times, peak heights and peak areas were better than 1.6, 6.7 and 6.0%, respectively

AB - A demountable direct injection high efficiency nebulizer operating at low sample uptake rates was developed and used for coupling of capillary electrophoresis (CE) with inductively coupled plasma mass spectrometry (ICP-MS). When the nebulizer was used for continuous sample introduction, detection limits of 20 and 1 ng L-1 were obtained for Se-82 and Rh-103, respectively, at sample uptake rates of 10-30 muL min(-1), based on three times the standard deviation of blank solution (3 sigma (b), n = 10). The nebulizer was used as part of the interface for coupling of CE with ICP-MS and applied for speciation of aqueous selenium standards. The interface was operated in the self-aspirating mode with a sheath liquid uptake of 10 muL min(-1). The CE-ICP-MS system resulted in baseline separation of selenate, selenite, selenocystine and selenomethionine within a total analysis time of 5.4 min. Detection limits were in the sub mug Se L-1 range, corresponding to absolute detection limits in the range 25-125 fg selenium. Repeatability (n = 6) expressed as relative standard deviations with respect to migration times, peak heights and peak areas were better than 1.6, 6.7 and 6.0%, respectively

M3 - Journal article

VL - 16

SP - 38

EP - 42

JO - Journal of Analytical Atomic Spectrometry

JF - Journal of Analytical Atomic Spectrometry

SN - 0267-9477

IS - 1

ER -