Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops

Research output: Contribution to journal › Journal article › peer-review

Standard

Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops. / Mullertz, Olivia; Hedengran, Anne; Mouhammad, Zaynab Ahmad; Freiberg, Josefine; Nagymihaly, Richard; Jacobsen, Jette; Larsen, Susan Weng; Bair, Jeffrey; Utheim, Tor; Dartt, Darlene A.; Heegaard, Steffen; Petrovski, Goran; Kolko, Miriam.

In: BMJ Open Ophthalmology, Vol. 6, No. 1, 000892, 2021.

Research output: Contribution to journal › Journal article › peer-review

Harvard

Mullertz, O, Hedengran, A, Mouhammad, ZA, Freiberg, J, Nagymihaly, R, Jacobsen, J, Larsen, SW, Bair, J, Utheim, T, Dartt, DA, Heegaard, S, Petrovski, G & Kolko, M 2021, 'Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops', BMJ Open Ophthalmology, vol. 6, no. 1, 000892. https://doi.org/10.1136/bmjophth-2021-000892

APA

Mullertz, O., Hedengran, A., Mouhammad, Z. A., Freiberg, J., Nagymihaly, R., Jacobsen, J., Larsen, S. W., Bair, J., Utheim, T., Dartt, D. A., Heegaard, S., Petrovski, G., & Kolko, M. (2021). Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops. BMJ Open Ophthalmology, 6(1), [000892]. https://doi.org/10.1136/bmjophth-2021-000892

Vancouver

Mullertz O, Hedengran A, Mouhammad ZA, Freiberg J, Nagymihaly R, Jacobsen J et al. Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops. BMJ Open Ophthalmology. 2021;6(1). 000892. https://doi.org/10.1136/bmjophth-2021-000892

Author

Mullertz, Olivia ; Hedengran, Anne ; Mouhammad, Zaynab Ahmad ; Freiberg, Josefine ; Nagymihaly, Richard ; Jacobsen, Jette ; Larsen, Susan Weng ; Bair, Jeffrey ; Utheim, Tor ; Dartt, Darlene A. ; Heegaard, Steffen ; Petrovski, Goran ; Kolko, Miriam. / Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops. In: BMJ Open Ophthalmology. 2021 ; Vol. 6, No. 1.

Bibtex

@article{53179d7f0dd44b17bad680c4c49e888f,
title = "Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops",
abstract = "Objective To investigate the short-term impact on human conjunctival goblet cell (GC) survival and mucin release of acute exposure to benzalkonium chloride (BAK) preserved and preservative-free (PF) 0.005% (w/v) latanoprost (LT) eye drops, and to compare the eye drops' physicochemical properties.Methods and analysis Primary GC cultures were established from human conjunctival donor tissue. The impact of eye drops on GC survival was assessed using a lactate dehydrogenase assay. Mucin release was evaluated through mucin-specific immunostaining. pH value, osmolality, drop mass and surface tension for all LT eye drops were measured.Results After application with PF-LT for 30 min (min), the GC survival was maintained compared with control (p=0.9941), while all BAK-LT eye drops reduced survival with approximately 30% (pConclusion PF-LT compared with various branded and generic LT preparations containing BAK are less cytotoxic when applied to cultured GCs.",
keywords = "TEAR FILM STABILITY, OPEN-ANGLE GLAUCOMA, BRANDED LATANOPROST, OCULAR HYPERTENSION, PHYSICAL-PROPERTIES, THERAPY, SURFACE, VIVO",
author = "Olivia Mullertz and Anne Hedengran and Mouhammad, {Zaynab Ahmad} and Josefine Freiberg and Richard Nagymihaly and Jette Jacobsen and Larsen, {Susan Weng} and Jeffrey Bair and Tor Utheim and Dartt, {Darlene A.} and Steffen Heegaard and Goran Petrovski and Miriam Kolko",
year = "2021",
doi = "10.1136/bmjophth-2021-000892",
language = "English",
volume = "6",
journal = "BMJ Open Ophthalmology",
issn = "2397-3269",
publisher = "BMJ Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Impact of benzalkonium chloride-preserved and preservative-free latanoprost eye drops on cultured human conjunctival goblet cells upon acute exposure and differences in physicochemical properties of the eye drops

AU - Mullertz, Olivia

AU - Hedengran, Anne

AU - Mouhammad, Zaynab Ahmad

AU - Freiberg, Josefine

AU - Nagymihaly, Richard

AU - Jacobsen, Jette

AU - Larsen, Susan Weng

AU - Bair, Jeffrey

AU - Utheim, Tor

AU - Dartt, Darlene A.

AU - Heegaard, Steffen

AU - Petrovski, Goran

AU - Kolko, Miriam

PY - 2021

Y1 - 2021

N2 - Objective To investigate the short-term impact on human conjunctival goblet cell (GC) survival and mucin release of acute exposure to benzalkonium chloride (BAK) preserved and preservative-free (PF) 0.005% (w/v) latanoprost (LT) eye drops, and to compare the eye drops' physicochemical properties.Methods and analysis Primary GC cultures were established from human conjunctival donor tissue. The impact of eye drops on GC survival was assessed using a lactate dehydrogenase assay. Mucin release was evaluated through mucin-specific immunostaining. pH value, osmolality, drop mass and surface tension for all LT eye drops were measured.Results After application with PF-LT for 30 min (min), the GC survival was maintained compared with control (p=0.9941), while all BAK-LT eye drops reduced survival with approximately 30% (pConclusion PF-LT compared with various branded and generic LT preparations containing BAK are less cytotoxic when applied to cultured GCs.

AB - Objective To investigate the short-term impact on human conjunctival goblet cell (GC) survival and mucin release of acute exposure to benzalkonium chloride (BAK) preserved and preservative-free (PF) 0.005% (w/v) latanoprost (LT) eye drops, and to compare the eye drops' physicochemical properties.Methods and analysis Primary GC cultures were established from human conjunctival donor tissue. The impact of eye drops on GC survival was assessed using a lactate dehydrogenase assay. Mucin release was evaluated through mucin-specific immunostaining. pH value, osmolality, drop mass and surface tension for all LT eye drops were measured.Results After application with PF-LT for 30 min (min), the GC survival was maintained compared with control (p=0.9941), while all BAK-LT eye drops reduced survival with approximately 30% (pConclusion PF-LT compared with various branded and generic LT preparations containing BAK are less cytotoxic when applied to cultured GCs.

KW - TEAR FILM STABILITY

KW - OPEN-ANGLE GLAUCOMA

KW - BRANDED LATANOPROST

KW - OCULAR HYPERTENSION

KW - PHYSICAL-PROPERTIES

KW - THERAPY

KW - SURFACE

KW - VIVO

U2 - 10.1136/bmjophth-2021-000892

DO - 10.1136/bmjophth-2021-000892

M3 - Journal article

C2 - 34993350

VL - 6

JO - BMJ Open Ophthalmology

JF - BMJ Open Ophthalmology

SN - 2397-3269

IS - 1

M1 - 000892

ER -

ID: 288782911