Imaging of plant materials using indirect desorption electrospray ionization mass spectrometry
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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Imaging of plant materials using indirect desorption electrospray ionization mass spectrometry. / Janfelt, Christian.
Mass Spectrometry Imaging of Small Molecules. ed. / Lin He. Vol. 1203 Humana Press, 2015. p. 91-7 (Methods in Molecular Biology).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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TY - CHAP
T1 - Imaging of plant materials using indirect desorption electrospray ionization mass spectrometry
AU - Janfelt, Christian
PY - 2015
Y1 - 2015
N2 - Indirect desorption electrospray ionization mass spectrometry (DESI-MS) imaging is a method for imaging distributions of metabolites in plant materials, in particular leaves and petals. The challenge in direct imaging of such plant materials with DESI-MS is particularly the protective layer of cuticular wax present in leaves and petals. The cuticle protects the plant from drying out, but also makes it difficult for the DESI sprayer to reach the analytes of interest inside the plant material. A solution to this problem is to imprint the plant material onto a surface, thus releasing the analytes of interest from parts of their matrix while preserving the spatial information in the two dimensions. The imprint can then easily be imaged by DESI-MS. The method delivers simple and robust mass spectrometry imaging of plant material with very high success ratios.
AB - Indirect desorption electrospray ionization mass spectrometry (DESI-MS) imaging is a method for imaging distributions of metabolites in plant materials, in particular leaves and petals. The challenge in direct imaging of such plant materials with DESI-MS is particularly the protective layer of cuticular wax present in leaves and petals. The cuticle protects the plant from drying out, but also makes it difficult for the DESI sprayer to reach the analytes of interest inside the plant material. A solution to this problem is to imprint the plant material onto a surface, thus releasing the analytes of interest from parts of their matrix while preserving the spatial information in the two dimensions. The imprint can then easily be imaged by DESI-MS. The method delivers simple and robust mass spectrometry imaging of plant material with very high success ratios.
U2 - 10.1007/978-1-4939-1357-2_9
DO - 10.1007/978-1-4939-1357-2_9
M3 - Book chapter
C2 - 25361669
SN - 978-1-4939-1356-5
VL - 1203
T3 - Methods in Molecular Biology
SP - 91
EP - 97
BT - Mass Spectrometry Imaging of Small Molecules
A2 - He, Lin
PB - Humana Press
ER -
ID: 162123018