Hyaluronic acid-based nanogels improve in vivo compatibility of the anti-biofilm peptide DJK-5

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Hyaluronic acid-based nanogels improve in vivo compatibility of the anti-biofilm peptide DJK-5. / Kłodzińska, Sylvia N.; Pletzer, Daniel; Rahanjam, Negin; Rades, Thomas; Hancock, Robert E.W.; Nielsen, Hanne M.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 20, 102022, 2019.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kłodzińska, SN, Pletzer, D, Rahanjam, N, Rades, T, Hancock, REW & Nielsen, HM 2019, 'Hyaluronic acid-based nanogels improve in vivo compatibility of the anti-biofilm peptide DJK-5', Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 20, 102022. https://doi.org/10.1016/j.nano.2019.102022

APA

Kłodzińska, S. N., Pletzer, D., Rahanjam, N., Rades, T., Hancock, R. E. W., & Nielsen, H. M. (2019). Hyaluronic acid-based nanogels improve in vivo compatibility of the anti-biofilm peptide DJK-5. Nanomedicine: Nanotechnology, Biology, and Medicine, 20, [102022]. https://doi.org/10.1016/j.nano.2019.102022

Vancouver

Kłodzińska SN, Pletzer D, Rahanjam N, Rades T, Hancock REW, Nielsen HM. Hyaluronic acid-based nanogels improve in vivo compatibility of the anti-biofilm peptide DJK-5. Nanomedicine: Nanotechnology, Biology, and Medicine. 2019;20. 102022. https://doi.org/10.1016/j.nano.2019.102022

Author

Kłodzińska, Sylvia N. ; Pletzer, Daniel ; Rahanjam, Negin ; Rades, Thomas ; Hancock, Robert E.W. ; Nielsen, Hanne M. / Hyaluronic acid-based nanogels improve in vivo compatibility of the anti-biofilm peptide DJK-5. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2019 ; Vol. 20.

Bibtex

@article{9540ff1cc1b64ae6b4788b626d3e16d2,
title = "Hyaluronic acid-based nanogels improve in vivo compatibility of the anti-biofilm peptide DJK-5",
abstract = "Anti-biofilm peptides are a subset of antimicrobial peptides and represent promising broad-spectrum agents for the treatment of bacterial biofilms, though some display host toxicity in vivo. Here we evaluated nanogels composed of modified hyaluronic acid for the encapsulation of the anti-biofilm peptide DJK-5 in vivo. Nanogels of 174 to 194 nm encapsulating 33–60% of peptide were created. Efficacy and toxicity of the nanogels were tested in vivo employing a murine abscess model of a Pseudomonas aeruginosa LESB58 high bacterial density infection. The dose of DJK-5 that could be administered intravenously to mice without inducing toxicity was more than doubled after encapsulation in nanogels. Upon subcutaneous administration, the toxicity of the DJK-5 in nanogels was decreased four-fold compared to non-formulated peptide, without compromising the anti-abscess effect of DJK-5. These findings support the use of nanogels to increase the safety of antimicrobial and anti-biofilm peptides after intravenous and subcutaneous administration.",
keywords = "Biofilm, Cationic peptide, Drug delivery, Nanogel, Pseudomonas aeruginosa",
author = "K{\l}odzi{\'n}ska, {Sylvia N.} and Daniel Pletzer and Negin Rahanjam and Thomas Rades and Hancock, {Robert E.W.} and Nielsen, {Hanne M.}",
year = "2019",
doi = "10.1016/j.nano.2019.102022",
language = "English",
volume = "20",
journal = "Nanomedicine: Nanotechnology, Biology and Medicine",
issn = "1549-9634",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Hyaluronic acid-based nanogels improve in vivo compatibility of the anti-biofilm peptide DJK-5

AU - Kłodzińska, Sylvia N.

AU - Pletzer, Daniel

AU - Rahanjam, Negin

AU - Rades, Thomas

AU - Hancock, Robert E.W.

AU - Nielsen, Hanne M.

PY - 2019

Y1 - 2019

N2 - Anti-biofilm peptides are a subset of antimicrobial peptides and represent promising broad-spectrum agents for the treatment of bacterial biofilms, though some display host toxicity in vivo. Here we evaluated nanogels composed of modified hyaluronic acid for the encapsulation of the anti-biofilm peptide DJK-5 in vivo. Nanogels of 174 to 194 nm encapsulating 33–60% of peptide were created. Efficacy and toxicity of the nanogels were tested in vivo employing a murine abscess model of a Pseudomonas aeruginosa LESB58 high bacterial density infection. The dose of DJK-5 that could be administered intravenously to mice without inducing toxicity was more than doubled after encapsulation in nanogels. Upon subcutaneous administration, the toxicity of the DJK-5 in nanogels was decreased four-fold compared to non-formulated peptide, without compromising the anti-abscess effect of DJK-5. These findings support the use of nanogels to increase the safety of antimicrobial and anti-biofilm peptides after intravenous and subcutaneous administration.

AB - Anti-biofilm peptides are a subset of antimicrobial peptides and represent promising broad-spectrum agents for the treatment of bacterial biofilms, though some display host toxicity in vivo. Here we evaluated nanogels composed of modified hyaluronic acid for the encapsulation of the anti-biofilm peptide DJK-5 in vivo. Nanogels of 174 to 194 nm encapsulating 33–60% of peptide were created. Efficacy and toxicity of the nanogels were tested in vivo employing a murine abscess model of a Pseudomonas aeruginosa LESB58 high bacterial density infection. The dose of DJK-5 that could be administered intravenously to mice without inducing toxicity was more than doubled after encapsulation in nanogels. Upon subcutaneous administration, the toxicity of the DJK-5 in nanogels was decreased four-fold compared to non-formulated peptide, without compromising the anti-abscess effect of DJK-5. These findings support the use of nanogels to increase the safety of antimicrobial and anti-biofilm peptides after intravenous and subcutaneous administration.

KW - Biofilm

KW - Cationic peptide

KW - Drug delivery

KW - Nanogel

KW - Pseudomonas aeruginosa

U2 - 10.1016/j.nano.2019.102022

DO - 10.1016/j.nano.2019.102022

M3 - Journal article

C2 - 31170510

AN - SCOPUS:85068142260

VL - 20

JO - Nanomedicine: Nanotechnology, Biology and Medicine

JF - Nanomedicine: Nanotechnology, Biology and Medicine

SN - 1549-9634

M1 - 102022

ER -

ID: 226872960